LOCUS       sequence-471448-        2579 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  rpoC1 5'UTR Chlamydomonas reinhardtii.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_3_020_rpoC1_5'UTR_Cr
FEATURES             Location/Qualifiers
     source          1..2579
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      136..230
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      413..1001
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     902..921
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1155..1172
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1196..1253)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    1784..1805
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      1808..1851
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(1855..1873)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             join(2035..2579,1..115)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2101..2120)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg
       61 cagaatgctt aatgaattac aacagtactg cgacgagtgg cagggcgggg cgtaatttga
      121 tatcgagctc gcttggactc ctgttgatag atccagtaat gacctcagaa ctccatctgg
      181 atttgttcag aacgctcggt tgccgccggg cgttttttat tggtgagaat ccaagcctcg
      241 agctgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
      301 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
      361 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
      421 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
      481 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
      541 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
      601 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
      661 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
      721 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
      781 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
      841 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
      901 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
      961 gatttttgtg atgctcgtca ggggggcgga gcctgtggaa aaacgccagc aacgcggcct
     1021 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     1081 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     1141 gaacgaccga gcgcagcgag tcagtgagcg aggaagcctg cataacgcga agtaatcttt
     1201 tcggttttaa agaaaaaggg cagggtggtg acaccttgcc cttttttgcc ggactgcagc
     1261 ggccgctact agtcgcgatg agtggtctca cattaataaa tattaaaagg attttcttgt
     1321 agcttaatgt ttaataaaat gcaattttat atggtagttc tatgtgggta aagtcctttg
     1381 aaactaatcc cgataaagca ttagatctca cacataaacg actgcggggc tacaaagtcg
     1441 aactaacata taaagcttta ttaagcttaa gattttttaa ccagaaaaaa ccatatttta
     1501 tgtatagcta acaaggctac ctcatttgga tatataaata acatggccgt gccagcccga
     1561 agggggacat tggttctgtc tcgaggaagt agtaacttat tacttactgt gtacctgtaa
     1621 ggggacgcca gttggtacat atacctgaag agggtagcag gtagtaacta caattcgctt
     1681 cgggtataat gaaccatttc ggggcccttt aagggttgat ataacatttt agtgaattta
     1741 tacatccgca attacataca ctatagtacg agaccactca tcgctctaga agcggccgcg
     1801 aattccagaa atcatcctta gcgaaagcta aggatttttt ttatctgaaa ttctgcctcg
     1861 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     1921 gcactcgagt tgatcgggca cgtaagaggt tccaactttc accataatga aataagatca
     1981 ctaccgggcg tattttttga gttatcgaga ttttcaggag ctaaggaagc taaaatggag
     2041 aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt
     2101 gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg
     2161 gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt
     2221 cttgcccgcc tgatgaatgc tcatccggaa tttcgtatgg caatgaaaga cggtgagctg
     2281 gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt
     2341 tcatcactct ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa
     2401 gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg
     2461 tttttcgtat cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat
     2521 atggacaact tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaa
//
