LOCUS       sequence-471449-        2397 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  tufA 5'UTR Chlamydomonas reinhardtii.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_3_023_tufA_5'UTR_Cr
FEATURES             Location/Qualifiers
     source          1..2397
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(159..253)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(274..933)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     848..867
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1095..1113
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1117..1160)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    1163..1184
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      1533..1590
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1614..1631)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1785..2373)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1865..1884)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
       61 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
      121 atatatgagt aaacttggtc tgacagctcg aggcttggat tctcaccaat aaaaaacgcc
      181 cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct
      241 atcaacagga gtccaagcga gctcgatatc aaattacgcc ccgccctgcc actcgtcgca
      301 gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaaa cggcatgatg
      361 aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt
      421 gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact
      481 cacccaggga ttggctgata cgaaaaacat attctcaata aaccctttag ggaaataggc
      541 caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc
      601 gtcgtggtat tcactccaga gtgatgaaaa cgtttcagtt tgctcatgga aaacggtgta
      661 acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca tacgaaattc
      721 cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt
      781 atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt
      841 acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc
      901 aacggtggta tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct
      961 cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc
     1021 tcttacgtgc ccgatcaact cgagtgccac ctgacgtcta agaaaccatt attatcatga
     1081 cattaaccta taaaaatagg cgtatcacga ggcagaattt cagataaaaa aaatccttag
     1141 ctttcgctaa ggatgatttc tggaattcgc ggccgcttct agagcgatga gtggtctcgt
     1201 actaacagaa ctactgtagt tttttaaatt atacctaaac ctgaaaaatt ggattatata
     1261 gcaataacac tattaagaac tatatattaa aatgtcaata ctatcccgta gggactatga
     1321 aactaaaaca cctatccgaa tgttaatgct tagtgagcaa agtatgcagt atttatctac
     1381 aaaaaaggag aaactcttac ctctggttag aggtttcttt tttaatgttt gaagcattaa
     1441 caatctaatt ccaaaagaag tgactaaata ataatttatt aaataacaca taatgtgaga
     1501 ccactcatcg cgactagtag cggccgctgc agtccggcaa aaaagggcaa ggtgtcacca
     1561 ccctgccctt tttctttaaa accgaaaaga ttacttcgcg ttatgcaggc ttcctcgctc
     1621 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     1681 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     1741 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca caggctccgc
     1801 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     1861 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     1921 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     1981 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     2041 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     2101 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     2161 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     2221 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     2281 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     2341 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacg
//
