LOCUS       sequence-471450-        2325 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  clpP 5'UTR Chlamydomonas reinhardtii.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_3_024_clpP_5'UTR_Cr
FEATURES             Location/Qualifiers
     source          1..2325
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      178..235
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(259..276)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(430..1018)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(510..529)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1201..1295)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1316..1975)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1890..1909
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2137..2155
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2159..2202)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2205..2226
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 caaaggagcc tgtaaacggt actttaattt ttacagtagc actcgcagag cttatttacg
       61 tgcaaataaa agctctatct actaggatat tagactagta ttaataaaac acaacatttt
      121 attaacaaag taatttaatg tgagaccact catcgcgact agtagcggcc gctgcagtcc
      181 ggcaaaaaag ggcaaggtgt caccaccctg ccctttttct ttaaaaccga aaagattact
      241 tcgcgttatg caggcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
      301 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
      361 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
      421 gctggcgttt ttccacaggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
      481 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
      541 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
      601 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
      661 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
      721 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
      781 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
      841 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
      901 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
      961 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     1021 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     1081 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     1141 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagct cgaggcttgg
     1201 attctcacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag
     1261 ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgata tcaaattacg
     1321 ccccgccctg ccactcgtcg cagtactgtt gtaattcatt aagcattctg ccgacatgga
     1381 agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt
     1441 gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt
     1501 ttaaatcaaa actggtgaaa ctcacccagg gattggctga tacgaaaaac atattctcaa
     1561 taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata
     1621 tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagtgatgaa aacgtttcag
     1681 tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt
     1741 ctttcattgc catacgaaat tccggatgag cattcatcag gcgggcaaga atgtgaataa
     1801 aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca
     1861 gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt
     1921 tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag
     1981 cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt
     2041 cattatggtg aaagttggaa cctcttacgt gcccgatcaa ctcgagtgcc acctgacgtc
     2101 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcagaat
     2161 ttcagataaa aaaaatcctt agctttcgct aaggatgatt tctggaattc gcggccgctt
     2221 ctagagcgat gagtggtctc gtacttttaa gtttaacgta gagccaatat ttgtttgaat
     2281 ttatctattt tttaaaccat tttggtttaa aatttttatt tgctt
//
