LOCUS       sequence-471451-        2670 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  rpl16 5'UTR Chlamydomonas reinhardtii.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_3_026_rpl16_5'UTR_Cr
FEATURES             Location/Qualifiers
     source          1..2670
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      180..274
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      457..1045
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     946..965
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1199..1216
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1240..1297)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    1919..1940
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      1943..1986
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(1990..2008)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             join(2170..2670,1..159)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2236..2255)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg
       61 gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa
      121 ttacaacagt actgcgacga gtggcagggc ggggcgtaat ttgatatcga gctcgcttgg
      181 actcctgttg atagatccag taatgacctc agaactccat ctggatttgt tcagaacgct
      241 cggttgccgc cgggcgtttt ttattggtga gaatccaagc ctcgagctgt cagaccaagt
      301 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
      361 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
      421 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
      481 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
      541 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
      601 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
      661 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
      721 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
      781 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
      841 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
      901 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
      961 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     1021 gtcagggggg cggagcctgt ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     1081 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     1141 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     1201 cgagtcagtg agcgaggaag cctgcataac gcgaagtaat cttttcggtt ttaaagaaaa
     1261 agggcagggt ggtgacacct tgcccttttt tgccggactg cagcggccgc tactagtcgc
     1321 gatgagtggt ctcacattgt ttttatttat aattttaaat ttttatgtaa cagttaatta
     1381 aaaatttaat ttaaaagcta caaatacaca gaaatcaatt gtactttata ctagtaacta
     1441 gtaatatgta ttgatttttg tttatcttgc attaaagtgt acgtaccctt aacgggtacg
     1501 tacactttat acaatgaaca atactatcga tttttccgca attcttcagc aaaaagatag
     1561 atatttttag attttacttt aattgttctt ctgctggagt atattaatat aaaccgttgg
     1621 ccaagtgtac ttatctcgct tataggctaa caagtccctg ctaactgagt ataacgtgta
     1681 cgtacccgta aggatacgta cacgttatac tctagaatag ctatggctga cttaaattta
     1741 tttacctcgc ctataggcta acccttacag gtaaataaat atcaagttag gacatttatg
     1801 tacccgtaag ggggcagtgg cgagccactg cctcctttgg cagcgttcat gggcataaat
     1861 ttattaaaac agaatactta tggtaaaatt agtttaatca gtacgagacc actcatcgct
     1921 ctagaagcgg ccgcgaattc cagaaatcat ccttagcgaa agctaaggat tttttttatc
     1981 tgaaattctg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     2041 cttagacgtc aggtggcact cgagttgatc gggcacgtaa gaggttccaa ctttcaccat
     2101 aatgaaataa gatcactacc gggcgtattt tttgagttat cgagattttc aggagctaag
     2161 gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat
     2221 cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt
     2281 cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg
     2341 gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaatttcg tatggcaatg
     2401 aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag
     2461 caaactgaaa cgttttcatc actctggagt gaataccacg acgatttccg gcagtttcta
     2521 cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg
     2581 tttattgaga atatgttttt cgtatcagcc aatccctggg tgagtttcac cagttttgat
     2641 ttaaacgtgg ccaatatgga caacttcttc
//
