LOCUS       sequence-471452-        2150 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  atpH 5'UTR Chlamydomonas reinhardtii.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_3_030_atpH_5'UTR_Cr
FEATURES             Location/Qualifiers
     source          1..2150
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      197..785
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     686..705
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     939..956
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(980..1037)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    1139..1160
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      1163..1206
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(1210..1228)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             1390..2049
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1456..1475)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      join(2070..2150,1..14)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 ttattggtga gaatccaagc ctcgagctgt cagaccaagt ttactcatat atactttaga
       61 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
      121 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
      181 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
      241 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
      301 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
      361 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
      421 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
      481 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
      541 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
      601 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      661 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      721 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctgt
      781 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
      841 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
      901 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
      961 cctgcataac gcgaagtaat cttttcggtt ttaaagaaaa agggcagggt ggtgacacct
     1021 tgcccttttt tgccggactg cagcggccgc tactagtcgc gatgagtggt ctcacatttg
     1081 tatttcctcc taaatgaatc aataaaatcg ataacaacca gtacgagacc actcatcgct
     1141 ctagaagcgg ccgcgaattc cagaaatcat ccttagcgaa agctaaggat tttttttatc
     1201 tgaaattctg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     1261 cttagacgtc aggtggcact cgagttgatc gggcacgtaa gaggttccaa ctttcaccat
     1321 aatgaaataa gatcactacc gggcgtattt tttgagttat cgagattttc aggagctaag
     1381 gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat
     1441 cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt
     1501 cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg
     1561 gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaatttcg tatggcaatg
     1621 aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag
     1681 caaactgaaa cgttttcatc actctggagt gaataccacg acgatttccg gcagtttcta
     1741 cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg
     1801 tttattgaga atatgttttt cgtatcagcc aatccctggg tgagtttcac cagttttgat
     1861 ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat
     1921 acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat
     1981 ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgacga gtggcagggc
     2041 ggggcgtaat ttgatatcga gctcgcttgg actcctgttg atagatccag taatgacctc
     2101 agaactccat ctggatttgt tcagaacgct cggttgccgc cgggcgtttt
//
