LOCUS       sequence-471456-        2370 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  petA 5'UTR Chlamydomonas reinhardtii.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_3a_002_petA_5'UTR_Cr
FEATURES             Location/Qualifiers
     source          1..2370
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      31..125
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      308..896
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     797..816
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1050..1067
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1091..1148)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    1470..1491
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      1494..1537
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(1541..1559)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             join(1721..2370,1..10)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1787..1806)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 cggggcgtaa tttgatatcg agctcgcttg gactcctgtt gatagatcca gtaatgacct
       61 cagaactcca tctggatttg ttcagaacgc tcggttgccg ccgggcgttt tttattggtg
      121 agaatccaag cctcgagctg tcagaccaag tttactcata tatactttag attgatttaa
      181 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
      241 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
      301 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
      361 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
      421 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
      481 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
      541 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
      601 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
      661 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
      721 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
      781 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
      841 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagcctg tggaaaaacg
      901 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
      961 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     1021 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcctgcataa
     1081 cgcgaagtaa tcttttcggt tttaaagaaa aagggcaggg tggtgacacc ttgccctttt
     1141 ttgccggact gcagcggccg ctactagtcg cgatgagtgg tctcaatgga attttattaa
     1201 tcttaaaacg aaactagaat aaaaactatt caattttaaa atagatatta atttatctat
     1261 aaggggattc cagttgcttt tcaactggac tcctatttta aaatagaatt ttttcgtgga
     1321 taaaccccta cccatacggg tacgaggaaa gtgcaactaa gcagcctcaa caagtttatt
     1381 ctagctgaaa aagtaagcat aagtaattta cattcataat tactatttaa attttatttt
     1441 ttttcttctc agtacgagac cactcatcgc tctagaagcg gccgcgaatt ccagaaatca
     1501 tccttagcga aagctaagga ttttttttat ctgaaattct gcctcgtgat acgcctattt
     1561 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac tcgagttgat
     1621 cgggcacgta agaggttcca actttcacca taatgaaata agatcactac cgggcgtatt
     1681 ttttgagtta tcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg
     1741 atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc
     1801 agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac
     1861 cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat
     1921 gaatgctcat ccggaatttc gtatggcaat gaaagacggt gagctggtga tatgggatag
     1981 tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat cactctggag
     2041 tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta
     2101 cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtatcagc
     2161 caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt
     2221 cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct
     2281 ggcgattcag gttcatcatg ccgtttgtga tggcttccat gtcggcagaa tgcttaatga
     2341 attacaacag tactgcgacg agtggcaggg
//
