LOCUS       sequence-471458-        2179 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  psbE 5'UTR  Chlamydomonas reinhardtii.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_3a_004_psbE_5'UTR_Cr
FEATURES             Location/Qualifiers
     source          1..2179
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(50..144)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(165..824)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     739..758
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     986..1004
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1008..1051)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    1054..1075
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      1206..1263
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1287..1304)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1458..2046)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1538..1557)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 caatctaaag tatatatgag taaacttggt ctgacagctc gaggcttgga ttctcaccaa
       61 taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca
      121 ttactggatc tatcaacagg agtccaagcg agctcgatat caaattacgc cccgccctgc
      181 cactcgtcgc agtactgttg taattcatta agcattctgc cgacatggaa gccatcacaa
      241 acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat
      301 ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa
      361 ctggtgaaac tcacccaggg attggctgat acgaaaaaca tattctcaat aaacccttta
      421 gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac
      481 tgccggaaat cgtcgtggta ttcactccag agtgatgaaa acgtttcagt ttgctcatgg
      541 aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc
      601 atacgaaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa
      661 aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc
      721 tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat
      781 tgggatatat caacggtggt atatccagtg atttttttct ccattttagc ttccttagct
      841 cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga
      901 aagttggaac ctcttacgtg cccgatcaac tcgagtgcca cctgacgtct aagaaaccat
      961 tattatcatg acattaacct ataaaaatag gcgtatcacg aggcagaatt tcagataaaa
     1021 aaaatcctta gctttcgcta aggatgattt ctggaattcg cggccgcttc tagagcgatg
     1081 agtggtctcg tactataata cattgattat aaagcagaca aattgttgaa aaagctaagt
     1141 aagcacactt aggaggtatt ttttccattg agaccactca tcgcgactag tagcggccgc
     1201 tgcagtccgg caaaaaaggg caaggtgtca ccaccctgcc ctttttcttt aaaaccgaaa
     1261 agattacttc gcgttatgca ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     1321 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     1381 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     1441 gccgcgttgc tggcgttttt ccacaggctc cgcccccctg acgagcatca caaaaatcga
     1501 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     1561 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     1621 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     1681 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     1741 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     1801 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     1861 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     1921 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     1981 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     2041 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     2101 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     2161 taaaaatgaa gttttaaat
//
