LOCUS       sequence-471459-        2201 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  rbcL 5'UTR Chlamydomonas reinhardtii.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_3a_005_rbcL_5'UTR_Cr
FEATURES             Location/Qualifiers
     source          1..2201
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      220..808
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     709..728
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     962..979
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1003..1060)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    1213..1234
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      1237..1280
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(1284..1302)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             1464..2123
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1530..1549)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      join(2144..2201,1..37)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 gctcggttgc cgccgggcgt tttttattgg tgagaatcca agcctcgagc tgtcagacca
       61 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
      121 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
      181 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
      241 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
      301 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
      361 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
      421 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
      481 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
      541 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
      601 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
      661 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
      721 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
      781 ctcgtcaggg gggcggagcc tgtggaaaaa cgccagcaac gcggcctttt tacggttcct
      841 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
      901 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
      961 cagcgagtca gtgagcgagg aagcctgcat aacgcgaagt aatcttttcg gttttaaaga
     1021 aaaagggcag ggtggtgaca ccttgccctt ttttgccgga ctgcagcggc cgctactagt
     1081 cgcgatgagt ggtctcaatg gttatataaa taaatgtaac ttcttttgac gatcctaaaa
     1141 taatctgtcc ggaaatataa tttaaaaatt gttgaaaaat tttaaataca tttagtacga
     1201 gaccactcat cgctctagaa gcggccgcga attccagaaa tcatccttag cgaaagctaa
     1261 ggattttttt tatctgaaat tctgcctcgt gatacgccta tttttatagg ttaatgtcat
     1321 gataataatg gtttcttaga cgtcaggtgg cactcgagtt gatcgggcac gtaagaggtt
     1381 ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag ttatcgagat
     1441 tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata
     1501 tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct
     1561 ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc
     1621 acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat
     1681 ttcgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca
     1741 ccgttttcca tgagcaaact gaaacgtttt catcactctg gagtgaatac cacgacgatt
     1801 tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct
     1861 atttccctaa agggtttatt gagaatatgt ttttcgtatc agccaatccc tgggtgagtt
     1921 tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca
     1981 tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc
     2041 atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg
     2101 acgagtggca gggcggggcg taatttgata tcgagctcgc ttggactcct gttgatagat
     2161 ccagtaatga cctcagaact ccatctggat ttgttcagaa c
//
