LOCUS       sequence-471460-        2303 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  atpE-ycf12 IEE Chlamydomonas reinhardtii.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_3IEE_001_atpE-ycf12_IEE_Cr
FEATURES             Location/Qualifiers
     source          1..2303
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      275..863
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     764..783
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1017..1034
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1058..1115)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    1370..1391
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      1394..1437
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(1441..1459)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             1621..2280
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1687..1706)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      join(2301..2303,1..92)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc agaacgctcg
       61 gttgccgccg ggcgtttttt attggtgaga atccaagcct cgagctgtca gaccaagttt
      121 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
      181 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
      241 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
      301 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
      361 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
      421 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
      481 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
      541 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
      601 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
      661 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
      721 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
      781 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
      841 caggggggcg gagcctgtgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
      901 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
      961 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     1021 agtcagtgag cgaggaagcc tgcataacgc gaagtaatct tttcggtttt aaagaaaaag
     1081 ggcagggtgg tgacaccttg cccttttttg ccggactgca gcggccgcta ctagtcgcga
     1141 tgagtggtct cacattatga aaaatttttt tttatataca aaatcaaaaa gtaacaagaa
     1201 cataaacttt aaataatgtt agatctatgt atttacccaa agagtatact gttcaactct
     1261 atcagacttg aatagctcta gataatttta caaaccctct aataggaaca tatactactt
     1321 tcttattagg gatttgttga taagttgaaa agtacgagac cactcatcgc tctagaagcg
     1381 gccgcgaatt ccagaaatca tccttagcga aagctaagga ttttttttat ctgaaattct
     1441 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     1501 caggtggcac tcgagttgat cgggcacgta agaggttcca actttcacca taatgaaata
     1561 agatcactac cgggcgtatt ttttgagtta tcgagatttt caggagctaa ggaagctaaa
     1621 atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa
     1681 cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat
     1741 attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt
     1801 cacattcttg cccgcctgat gaatgctcat ccggaatttc gtatggcaat gaaagacggt
     1861 gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa
     1921 acgttttcat cactctggag tgaataccac gacgatttcc ggcagtttct acacatatat
     1981 tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag
     2041 aatatgtttt tcgtatcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg
     2101 gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc
     2161 gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga tggcttccat
     2221 gtcggcagaa tgcttaatga attacaacag tactgcgacg agtggcaggg cggggcgtaa
     2281 tttgatatcg agctcgcttg gac
//
