LOCUS       sequence-471462-        2963 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  psbE-rps9 IEE Chlamydomonas reinhardtii.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_3IEE_008_psbE-rps9_IEE_Cr
FEATURES             Location/Qualifiers
     source          1..2963
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      328..422
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      605..1193
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1094..1113
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1347..1364
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1388..1445)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    2360..2381
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      2384..2427
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(2431..2449)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             join(2611..2963,1..307)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2677..2696)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 catatattcg caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt
       61 tattgagaat atgtttttcg tatcagccaa tccctgggtg agtttcacca gttttgattt
      121 aaacgtggcc aatatggaca acttcttcgc ccccgttttc accatgggca aatattatac
      181 gcaaggcgac aaggtgctga tgccgctggc gattcaggtt catcatgccg tttgtgatgg
      241 cttccatgtc ggcagaatgc ttaatgaatt acaacagtac tgcgacgagt ggcagggcgg
      301 ggcgtaattt gatatcgagc tcgcttggac tcctgttgat agatccagta atgacctcag
      361 aactccatct ggatttgttc agaacgctcg gttgccgccg ggcgtttttt attggtgaga
      421 atccaagcct cgagctgtca gaccaagttt actcatatat actttagatt gatttaaaac
      481 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
      541 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
      601 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
      661 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
      721 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
      781 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
      841 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
      901 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
      961 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     1021 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     1081 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     1141 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctgtgg aaaaacgcca
     1201 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     1261 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     1321 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcc tgcataacgc
     1381 gaagtaatct tttcggtttt aaagaaaaag ggcagggtgg tgacaccttg cccttttttg
     1441 ccggactgca gcggccgcta ctagtcgcga tgagtggtct cacattacaa aaataaattt
     1501 ttaatttgct ttatttctat attttttaaa agacatccga cacccctttt aaaatacaac
     1561 ataataccta aaagcaactg aaaaaacaat gagagatttt ttcatctttt gctaaagcca
     1621 tattacactt cccatataaa attttttatg ctctccttct ggtaagtaac ctatttttta
     1681 ggttatatga ctgatttcgg ccaataaatt ttaataggag tttcaaatgt attgtacata
     1741 tcgggaaaat gcttgtcaca aatttttaat tcgcacatgc gttaaattat caaaaatcgt
     1801 ctttcaaaaa tataaatgtt accatttaaa ttgaaattaa actcacaagc aatcaaaata
     1861 aactataaaa aatattttgc aaataaacac aagggaattt agtttcctgt tgagatttat
     1921 gattctgtta agaggaaaat ggacgatatt cattgtaaaa cctaccatat cacaaagtct
     1981 agaaagtgcg ctaaaagcac aatgattaga actaatcttt taccctatta gtcatctaga
     2041 ctagtgccag ttgctcatgt ttatttatga acaatatttt tttaaaatca ctaaaaagct
     2101 ttctttatct ttatggaaac tcctatatat atcttatatt actatcatat tattatttct
     2161 tgtacgtgag ttatgctaac tgacatcaat ggactttttc tttaggattt acgttttttt
     2221 taatcattat gtgtggtaga cccaaattaa atgcctttgc caagatttat atagatggtt
     2281 tgaaaaggta ctttttttac ttagaaaaaa ttttctaagt aaaaaaagtt tttagattaa
     2341 agtacgagac cactcatcgc tctagaagcg gccgcgaatt ccagaaatca tccttagcga
     2401 aagctaagga ttttttttat ctgaaattct gcctcgtgat acgcctattt ttataggtta
     2461 atgtcatgat aataatggtt tcttagacgt caggtggcac tcgagttgat cgggcacgta
     2521 agaggttcca actttcacca taatgaaata agatcactac cgggcgtatt ttttgagtta
     2581 tcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg atataccacc
     2641 gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa
     2701 tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa
     2761 aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat
     2821 ccggaatttc gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct
     2881 tgttacaccg ttttccatga gcaaactgaa acgttttcat cactctggag tgaataccac
     2941 gacgatttcc ggcagtttct aca
//
