LOCUS       sequence-471463-        2894 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  rpl2-rps19 IEE Chlamydomonas reinhardtii.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_3IEE_013_rpl2-rps19_IEE_Cr
FEATURES             Location/Qualifiers
     source          1..2894
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    786..807
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      810..853
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(857..875)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             1037..1696
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1103..1122)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1717..1811
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      1994..2582
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2483..2502
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2736..2753
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2777..2834)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
ORIGIN
        1 tttgaatttt ataataatgg ctaaagctgt atttataaaa ataaaagtag gtaatatttt
       61 ctattcatgt ttaacaattg tctattttaa gtacatatga gctttttaaa ttccacatta
      121 aattgaaatt tattggaatt tttccaacat atgaccatta tagtggttta cccaatatag
      181 agttaagata tataaactct aaggataaac aaaacgaacc aatcagttga aacaaatgtt
      241 tatctgaaac tggttgtttt tagcgacttt agacttacta agtcccgata tctataaagt
      301 ttaaacaaac aattaacgtt aaaaatttat caaccaaaac tttattattt ctagaatttt
      361 tttaatattg taaaatttaa gcaagtggcc atacttatgc ttactgctat tttacgtccc
      421 tttacaggac aaatttattt attgtccccg agaaagaata aattttagtg gacgtcagtg
      481 gcagttgcct gccaactgcc gatatttata tactatttat ttattgtacc actgccacta
      541 atatttatat tcccgtaagg ggacgtcctc cttccccttc gggcaagtaa acttaggagt
      601 atgtaaacct gctagcgcag caaataaatt ttattctaag tttacttgcc cgaaggggaa
      661 ggaggcagtt gcctgccaac aaatttattt attgtcccgt aaggggaagg gggtaaataa
      721 atagccaaaa gaggccactt gttttaattg acctatcttt aattagagta cgagaccact
      781 catcgctcta gaagcggccg cgaattccag aaatcatcct tagcgaaagc taaggatttt
      841 ttttatctga aattctgcct cgtgatacgc ctatttttat aggttaatgt catgataata
      901 atggtttctt agacgtcagg tggcactcga gttgatcggg cacgtaagag gttccaactt
      961 tcaccataat gaaataagat cactaccggg cgtatttttt gagttatcga gattttcagg
     1021 agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca
     1081 atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca
     1141 gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt
     1201 ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg aatttcgtat
     1261 ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt
     1321 ccatgagcaa actgaaacgt tttcatcact ctggagtgaa taccacgacg atttccggca
     1381 gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc
     1441 taaagggttt attgagaata tgtttttcgt atcagccaat ccctgggtga gtttcaccag
     1501 ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa
     1561 atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt
     1621 ttgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact gcgacgagtg
     1681 gcagggcggg gcgtaatttg atatcgagct cgcttggact cctgttgata gatccagtaa
     1741 tgacctcaga actccatctg gatttgttca gaacgctcgg ttgccgccgg gcgtttttta
     1801 ttggtgagaa tccaagcctc gagctgtcag accaagttta ctcatatata ctttagattg
     1861 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     1921 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     1981 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     2041 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     2101 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     2161 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     2221 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     2281 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     2341 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     2401 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     2461 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     2521 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctgtgga
     2581 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     2641 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     2701 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcct
     2761 gcataacgcg aagtaatctt ttcggtttta aagaaaaagg gcagggtggt gacaccttgc
     2821 ccttttttgc cggactgcag cggccgctac tagtcgcgat gagtggtctc acattttgat
     2881 tttttaaaat gact
//
