LOCUS       sequence-471464-        2836 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  rpl23-rpl2 IEE Chlamydomonas reinhardtii.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_3IEE_015_rpl23-rpl2_IEE_Cr
FEATURES             Location/Qualifiers
     source          1..2836
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      92..186
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      369..957
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     858..877
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1111..1128
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1152..1209)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    1997..2018
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      2021..2064
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(2068..2086)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             join(2248..2836,1..71)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2314..2333)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgac gagtggcagg
       61 gcggggcgta atttgatatc gagctcgctt ggactcctgt tgatagatcc agtaatgacc
      121 tcagaactcc atctggattt gttcagaacg ctcggttgcc gccgggcgtt ttttattggt
      181 gagaatccaa gcctcgagct gtcagaccaa gtttactcat atatacttta gattgattta
      241 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
      301 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
      361 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
      421 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
      481 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
      541 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
      601 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
      661 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      721 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
      781 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      841 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      901 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct gtggaaaaac
      961 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     1021 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     1081 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcctgcata
     1141 acgcgaagta atcttttcgg ttttaaagaa aaagggcagg gtggtgacac cttgcccttt
     1201 tttgccggac tgcagcggcc gctactagtc gcgatgagtg gtctcacatt aattatgttt
     1261 ttaagttgtt ttactattta tagattaggt actaataaaa tttacagcct gtctttcagc
     1321 tctcatgagg agtcaaacaa ttaaaagtgg accactaacg aacctcgttc gagctcctcc
     1381 ctttcccctt acgggacgcc agtggcggta acattgccac taaaattcat ttgcctcagc
     1441 agttggcagg acgtcccttt ccccttacgg gacgtcagtg gcagttgcct gccaactgcc
     1501 gatatttatc tactggcgtc ccgcagggga aaagggaaag gtgtttttca actacgcatt
     1561 ggcaccttaa acgggccttt gcatattaaa gggtttagcc gatagatagg ttaccactta
     1621 atagctagat ttattattag aaaaataacc ctttttatta aaaaattaag tatatttttt
     1681 atttacaatt ttatttatat gatacttgct tctaaaataa gcaaaagatt actataccgg
     1741 taaagggata cactgtaaag tctactgatt ccccttgagg ggcggcatga cagtgatttt
     1801 tggatagatc ctagagcttt ggctatgtat atccctaaac tacaaaagct gtcatttctg
     1861 gtacaatgtt tctctgcctc tttgggcaaa taaacttaga gtttaaaaat atcttttgtt
     1921 gttcttgaga ggacgccagt ggcagtggta ccgccactgc ctattttaaa tcagccaagt
     1981 acgagaccac tcatcgctct agaagcggcc gcgaattcca gaaatcatcc ttagcgaaag
     2041 ctaaggattt tttttatctg aaattctgcc tcgtgatacg cctattttta taggttaatg
     2101 tcatgataat aatggtttct tagacgtcag gtggcactcg agttgatcgg gcacgtaaga
     2161 ggttccaact ttcaccataa tgaaataaga tcactaccgg gcgtattttt tgagttatcg
     2221 agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt
     2281 gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt
     2341 acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat
     2401 aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg
     2461 gaatttcgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt
     2521 tacaccgttt tccatgagca aactgaaacg ttttcatcac tctggagtga ataccacgac
     2581 gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg
     2641 gcctatttcc ctaaagggtt tattgagaat atgtttttcg tatcagccaa tccctgggtg
     2701 agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc
     2761 accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt
     2821 catcatgccg tttgtg
//
