LOCUS       sequence-471465-        2471 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ycf3-rps18 IEE Chlamydomonas reinhardtii.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_3IEE_021_ycf3-rps18_IEE_Cr
FEATURES             Location/Qualifiers
     source          1..2471
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      82..176
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      359..947
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     848..867
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1101..1118
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1142..1199)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    1622..1643
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      1646..1689
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(1693..1711)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             join(1873..2471,1..61)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1939..1958)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tgtcggcaga atgcttaatg aattacaaca gtactgcgac gagtggcagg gcggggcgta
       61 atttgatatc gagctcgctt ggactcctgt tgatagatcc agtaatgacc tcagaactcc
      121 atctggattt gttcagaacg ctcggttgcc gccgggcgtt ttttattggt gagaatccaa
      181 gcctcgagct gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
      241 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
      301 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
      361 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
      421 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
      481 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
      541 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
      601 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
      661 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
      721 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
      781 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
      841 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
      901 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct gtggaaaaac gccagcaacg
      961 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     1021 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     1081 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcctgcata acgcgaagta
     1141 atcttttcgg ttttaaagaa aaagggcagg gtggtgacac cttgcccttt tttgccggac
     1201 tgcagcggcc gctactagtc gcgatgagtg gtctcacatt aaataaataa aaatttaaaa
     1261 tataaattgc gaaatactac ttagtaacta aagtagtttt ttataaaatt tgaagcagct
     1321 tagaccattg agaatacagc ttctccataa agcatattaa tcttgctgtt gtacagcccc
     1381 aaaggaaaat atctaaacct tttcttctta ggaacaccag cggcagtggt ggtaccactg
     1441 ccgctggcgg cctaggtaaa taaaattgcc actgaggggc tgcaatttgt tttaatttat
     1501 atcaataggt atttatattt tccttacaaa gtattaaaat atggcttaga gaagagtaaa
     1561 aatcaatctg gcaacttcaa gtcaaaaggg tcaacatctt ttagtacgag accactcatc
     1621 gctctagaag cggccgcgaa ttccagaaat catccttagc gaaagctaag gatttttttt
     1681 atctgaaatt ctgcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     1741 tttcttagac gtcaggtggc actcgagttg atcgggcacg taagaggttc caactttcac
     1801 cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt ttcaggagct
     1861 aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg
     1921 catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc
     1981 gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat
     2041 ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt tcgtatggca
     2101 atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat
     2161 gagcaaactg aaacgttttc atcactctgg agtgaatacc acgacgattt ccggcagttt
     2221 ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa
     2281 gggtttattg agaatatgtt tttcgtatca gccaatccct gggtgagttt caccagtttt
     2341 gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat
     2401 tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtttgt
     2461 gatggcttcc a
//
