LOCUS       sequence-471466-        2288 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ycf4-ycf3 IEE Chlamydomonas reinhardtii.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_3IEE_022_ycf4-ycf3_IEE_Cr
FEATURES             Location/Qualifiers
     source          1..2288
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      266..854
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     755..774
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1008..1025
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1049..1106)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    1346..1367
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      1370..1413
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(1417..1435)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             1597..2256
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1663..1682)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      join(2277..2288,1..83)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 tagatccagt aatgacctca gaactccatc tggatttgtt cagaacgctc ggttgccgcc
       61 gggcgttttt tattggtgag aatccaagcc tcgagctgtc agaccaagtt tactcatata
      121 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
      181 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
      241 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
      301 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
      361 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
      421 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
      481 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
      541 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
      601 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
      661 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
      721 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
      781 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
      841 ggagcctgtg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
      901 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
      961 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     1021 gcgaggaagc ctgcataacg cgaagtaatc ttttcggttt taaagaaaaa gggcagggtg
     1081 gtgacacctt gccctttttt gccggactgc agcggccgct actagtcgcg atgagtggtc
     1141 tcacatttaa aaataaaatc agcgatttat tgaaactaag tacttataac tataacaatt
     1201 acatttttgt actgtagtaa tattaggcaa cgtgcctttt aagctgcgcc aaaggtgcag
     1261 cttaataaca tctgtttaga cgttttgttt ttaatttaat ctctataaaa ttttaaaatc
     1321 agtattagta cgagaccact catcgctcta gaagcggccg cgaattccag aaatcatcct
     1381 tagcgaaagc taaggatttt ttttatctga aattctgcct cgtgatacgc ctatttttat
     1441 aggttaatgt catgataata atggtttctt agacgtcagg tggcactcga gttgatcggg
     1501 cacgtaagag gttccaactt tcaccataat gaaataagat cactaccggg cgtatttttt
     1561 gagttatcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat
     1621 accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt
     1681 gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta
     1741 aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat
     1801 gctcatccgg aatttcgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt
     1861 cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcact ctggagtgaa
     1921 taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt
     1981 gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt atcagccaat
     2041 ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc
     2101 cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg
     2161 attcaggttc atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta
     2221 caacagtact gcgacgagtg gcagggcggg gcgtaatttg atatcgagct cgcttggact
     2281 cctgttga
//
