LOCUS       sequence-471467-        2615 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  psbD promoter + 5'UTR Chlamydomonas reinhardtii.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_2-3_001_psbD_Promoter+5'UTR_Cr
FEATURES             Location/Qualifiers
     source          1..2615
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      150..244
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      427..1015
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     916..935
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1169..1186
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1210..1267)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    1834..1855
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      1858..1901
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(1905..1923)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             join(2085..2615,1..129)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2151..2170)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat
       61 ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgacga gtggcagggc
      121 ggggcgtaat ttgatatcga gctcgcttgg actcctgttg atagatccag taatgacctc
      181 agaactccat ctggatttgt tcagaacgct cggttgccgc cgggcgtttt ttattggtga
      241 gaatccaagc ctcgagctgt cagaccaagt ttactcatat atactttaga ttgatttaaa
      301 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
      361 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
      421 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
      481 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
      541 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
      601 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
      661 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
      721 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
      781 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
      841 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
      901 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
      961 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctgt ggaaaaacgc
     1021 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     1081 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     1141 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cctgcataac
     1201 gcgaagtaat cttttcggtt ttaaagaaaa agggcagggt ggtgacacct tgcccttttt
     1261 tgccggactg cagcggccgc tactagtcgc gatgagtggt ctcacatttg cgtgtatctc
     1321 caaaataaaa aaacaactca tcgttacgtt aaatttatta ttatttaatt ttaatcattg
     1381 tgtatttaat attataactt atataaaata aaattaaaaa taagcatttt ttacacacat
     1441 atttttaaat aaatctttaa acgggttata tatagttata tatatgggac tagaactgct
     1501 ttgtgcatag tcatcacaat tattatatta taaaccatga ataaaggttt tattattatg
     1561 atataaaaat gcataaaatt tttataaatt ttgcaagtaa aatatataat taggaaaaaa
     1621 tttaaaattt aaaatgttag tcaagtttac aactaatact tttaattttg tattttaagt
     1681 attggacatt tttgtggaat taaatgtacc aaatatccat ttaatttcat atgaatccta
     1741 aaaaaaatgt ttacttaaag ggtgtcaatc tgttacaata ttgatatact atgacgccag
     1801 tgacaattgc ctggctcccg agaccactca tcgctctaga agcggccgcg aattccagaa
     1861 atcatcctta gcgaaagcta aggatttttt ttatctgaaa ttctgcctcg tgatacgcct
     1921 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcactcgagt
     1981 tgatcgggca cgtaagaggt tccaactttc accataatga aataagatca ctaccgggcg
     2041 tattttttga gttatcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca
     2101 ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc
     2161 agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa
     2221 agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc
     2281 tgatgaatgc tcatccggaa tttcgtatgg caatgaaaga cggtgagctg gtgatatggg
     2341 atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcactct
     2401 ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt
     2461 gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtat
     2521 cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact
     2581 tcttcgcccc cgttttcacc atgggcaaat attat
//
