LOCUS       sequence-471469-        2615 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  psbD promoter + 5'UTR Chlamydomonas reinhardtii 2-3a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_2-3a_002_psbD_Promoter+5'UTR_Cr
FEATURES             Location/Qualifiers
     source          1..2615
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(264..358)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(379..1038)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     953..972
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1200..1218
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1222..1265)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    1268..1289
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      1856..1913
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1937..1954)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(2108..2615,1..81))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2188..2207)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
       61 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
      121 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
      181 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
      241 tggtctgaca gctcgaggct tggattctca ccaataaaaa acgcccggcg gcaaccgagc
      301 gttctgaaca aatccagatg gagttctgag gtcattactg gatctatcaa caggagtcca
      361 agcgagctcg atatcaaatt acgccccgcc ctgccactcg tcgcagtact gttgtaattc
      421 attaagcatt ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag
      481 cggcatcagc accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa
      541 gaagttgtcc atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc
      601 tgatacgaaa aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta
      661 acacgccaca tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact
      721 ccagagtgat gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact
      781 atcccatatc accagctcac cgtctttcat tgccatacga aattccggat gagcattcat
      841 caggcgggca agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt
      901 ctttaaaaag gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga
      961 ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc
     1021 agtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa
     1081 tacgcccggt agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgcccgat
     1141 caactcgagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     1201 ataggcgtat cacgaggcag aatttcagat aaaaaaaatc cttagctttc gctaaggatg
     1261 atttctggaa ttcgcggccg cttctagagc gatgagtggt ctcgggagcc aggcaattgt
     1321 cactggcgtc atagtatatc aatattgtaa cagattgaca ccctttaagt aaacattttt
     1381 tttaggattc atatgaaatt aaatggatat ttggtacatt taattccaca aaaatgtcca
     1441 atacttaaaa tacaaaatta aaagtattag ttgtaaactt gactaacatt ttaaatttta
     1501 aattttttcc taattatata ttttacttgc aaaatttata aaaattttat gcatttttat
     1561 atcataataa taaaaccttt attcatggtt tataatataa taattgtgat gactatgcac
     1621 aaagcagttc tagtcccata tatataacta tatataaccc gtttaaagat ttatttaaaa
     1681 atatgtgtgt aaaaaatgct tatttttaat tttattttat ataagttata atattaaata
     1741 cacaatgatt aaaattaaat aataataaat ttaacgtaac gatgagttgt ttttttattt
     1801 tggagataca cgcaccattg agaccactca tcgcgactag tagcggccgc tgcagtccgg
     1861 caaaaaaggg caaggtgtca ccaccctgcc ctttttcttt aaaaccgaaa agattacttc
     1921 gcgttatgca ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     1981 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     2041 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     2101 tggcgttttt ccacaggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     2161 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     2221 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     2281 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     2341 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     2401 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     2461 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     2521 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     2581 cagttacctt cggaaaaaga gttggtagct cttga
//
