LOCUS       sequence-471470-        2298 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  rbcL promoter + 5'UTR Chlamydomonas reinhardtii 2-3a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_2-3a_003_rbcL_Promoter+5'UTR_Cr
FEATURES             Location/Qualifiers
     source          1..2298
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      271..859
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     760..779
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1013..1030
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1054..1111)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    1361..1382
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      1385..1428
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(1432..1450)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             1612..2271
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1678..1697)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      join(2292..2298,1..88)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 gttgatagat ccagtaatga cctcagaact ccatctggat ttgttcagaa cgctcggttg
       61 ccgccgggcg ttttttattg gtgagaatcc aagcctcgag ctgtcagacc aagtttactc
      121 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
      181 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
      241 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
      301 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
      361 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
      421 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
      481 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
      541 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
      601 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      661 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      721 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      781 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      841 ggggcggagc ctgtggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      901 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      961 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     1021 agtgagcgag gaagcctgca taacgcgaag taatcttttc ggttttaaag aaaaagggca
     1081 gggtggtgac accttgccct tttttgccgg actgcagcgg ccgctactag tcgcgatgag
     1141 tggtctcaat ggttatataa ataaatgtaa cttcttttga cgatcctaaa ataatctgtc
     1201 cggaaatata atttaaaaat tgttgaaaaa ttttaaatac atttaaatat attatatatt
     1261 tagaaataaa aaataatgta aactagcaaa tttatgtact tgtcttatca gtcgagcata
     1321 gaaagggcct ttaggtatat gctcccgaga ccactcatcg ctctagaagc ggccgcgaat
     1381 tccagaaatc atccttagcg aaagctaagg atttttttta tctgaaattc tgcctcgtga
     1441 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     1501 ctcgagttga tcgggcacgt aagaggttcc aactttcacc ataatgaaat aagatcacta
     1561 ccgggcgtat tttttgagtt atcgagattt tcaggagcta aggaagctaa aatggagaaa
     1621 aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag
     1681 gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc
     1741 tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt
     1801 gcccgcctga tgaatgctca tccggaattt cgtatggcaa tgaaagacgg tgagctggtg
     1861 atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca
     1921 tcactctgga gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat
     1981 gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt
     2041 ttcgtatcag ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg
     2101 gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg
     2161 ctgatgccgc tggcgattca ggttcatcat gccgtttgtg atggcttcca tgtcggcaga
     2221 atgcttaatg aattacaaca gtactgcgac gagtggcagg gcggggcgta atttgatatc
     2281 gagctcgctt ggactcct
//
