LOCUS       sequence-471471-        2145 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  HiBiT N-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_3b_025_Hibit-tag
FEATURES             Location/Qualifiers
     source          1..2145
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(28..46)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             208..867
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(274..293)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      888..982
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      1165..1753
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1654..1673
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1907..1924
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1948..2005)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    2102..2123
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      join(2126..2145,1..24)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 ctaaggattt tttttatctg aaattctgcc tcgtgatacg cctattttta taggttaatg
       61 tcatgataat aatggtttct tagacgtcag gtggcactcg agttgatcgg gcacgtaaga
      121 ggttccaact ttcaccataa tgaaataaga tcactaccgg gcgtattttt tgagttatcg
      181 agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt
      241 gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt
      301 acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat
      361 aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg
      421 gaatttcgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt
      481 tacaccgttt tccatgagca aactgaaacg ttttcatcac tctggagtga ataccacgac
      541 gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg
      601 gcctatttcc ctaaagggtt tattgagaat atgtttttcg tatcagccaa tccctgggtg
      661 agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc
      721 accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt
      781 catcatgccg tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac
      841 tgcgacgagt ggcagggcgg ggcgtaattt gatatcgagc tcgcttggac tcctgttgat
      901 agatccagta atgacctcag aactccatct ggatttgttc agaacgctcg gttgccgccg
      961 ggcgtttttt attggtgaga atccaagcct cgagctgtca gaccaagttt actcatatat
     1021 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     1081 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     1141 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     1201 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     1261 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     1321 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     1381 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     1441 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     1501 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     1561 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     1621 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     1681 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     1741 gagcctgtgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     1801 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     1861 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     1921 cgaggaagcc tgcataacgc gaagtaatct tttcggtttt aaagaaaaag ggcagggtgg
     1981 tgacaccttg cccttttttg ccggactgca gcggccgcta ctagtcgcga tgagtggtct
     2041 cacattccag aaattttctt aaataaacgc caaccagaaa ccatggcgag accactcatc
     2101 gctctagaag cggccgcgaa ttccagaaat catccttagc gaaag
//
