LOCUS       sequence-471473-        2199 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  HA N-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_3b_027_HA-tag
FEATURES             Location/Qualifiers
     source          1..2199
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     190..207
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(231..288)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     CDS             complement(332..358)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(362..388)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(392..418)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    439..460
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      463..506
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(510..528)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             690..1349
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(756..775)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1370..1464
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      join(1647..2199,1..36)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2136..2155
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttgtgatgct cgtcaggggg gcggagcctg tggaaaaacg ccagcaacgc ggccttttta
       61 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
      121 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
      181 accgagcgca gcgagtcagt gagcgaggaa gcctgcataa cgcgaagtaa tcttttcggt
      241 tttaaagaaa aagggcaggg tggtgacacc ttgccctttt ttgccggact gcagcggccg
      301 ctactagtcg cgatgagtgg tctcacattc cagcataatc tggaacatca taaggataac
      361 cagcataatc aggaacatcg tatgggtatt ttgcgtaatc tggtacatca tatggataca
      421 tggcgagacc actcatcgct ctagaagcgg ccgcgaattc cagaaatcat ccttagcgaa
      481 agctaaggat tttttttatc tgaaattctg cctcgtgata cgcctatttt tataggttaa
      541 tgtcatgata ataatggttt cttagacgtc aggtggcact cgagttgatc gggcacgtaa
      601 gaggttccaa ctttcaccat aatgaaataa gatcactacc gggcgtattt tttgagttat
      661 cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg
      721 ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat
      781 gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa
      841 ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc
      901 cggaatttcg tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt
      961 gttacaccgt tttccatgag caaactgaaa cgttttcatc actctggagt gaataccacg
     1021 acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc
     1081 tggcctattt ccctaaaggg tttattgaga atatgttttt cgtatcagcc aatccctggg
     1141 tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt
     1201 tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg
     1261 ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa ttacaacagt
     1321 actgcgacga gtggcagggc ggggcgtaat ttgatatcga gctcgcttgg actcctgttg
     1381 atagatccag taatgacctc agaactccat ctggatttgt tcagaacgct cggttgccgc
     1441 cgggcgtttt ttattggtga gaatccaagc ctcgagctgt cagaccaagt ttactcatat
     1501 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     1561 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     1621 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     1681 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     1741 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     1801 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     1861 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     1921 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     1981 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     2041 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     2101 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     2161 cctgtcgggt ttcgccacct ctgacttgag cgtcgattt
//
