LOCUS       sequence-471475-        2145 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  HiBiT C-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_5a_003_Hibit-tag
FEATURES             Location/Qualifiers
     source          1..2145
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(20..39)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(711..805)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(826..1485)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1400..1419
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1647..1665
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1669..1712)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    1715..1736
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      1833..1890
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1914..1931)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(2085..2145,1..528))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
       61 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
      121 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
      181 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
      241 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
      301 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
      361 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
      421 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
      481 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
      541 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
      601 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
      661 tcaatctaaa gtatatatga gtaaacttgg tctgacagct cgaggcttgg attctcacca
      721 ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc
      781 attactggat ctatcaacag gagtccaagc gagctcgata tcaaattacg ccccgccctg
      841 ccactcgtcg cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca
      901 aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata
      961 tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa
     1021 actggtgaaa ctcacccagg gattggctga tacgaaaaac atattctcaa taaacccttt
     1081 agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa
     1141 ctgccggaaa tcgtcgtggt attcactcca gagtgatgaa aacgtttcag tttgctcatg
     1201 gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc
     1261 catacgaaat tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata
     1321 aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt
     1381 ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca
     1441 ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc
     1501 tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg
     1561 aaagttggaa cctcttacgt gcccgatcaa ctcgagtgcc acctgacgtc taagaaacca
     1621 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcagaat ttcagataaa
     1681 aaaaatcctt agctttcgct aaggatgatt tctggaattc gcggccgctt ctagagcgat
     1741 gagtggtctc ggctttagtt tctggttggc gtttatttaa gaaaatttct gggtatgaga
     1801 ccactcatcg cgactagtag cggccgctgc agtccggcaa aaaagggcaa ggtgtcacca
     1861 ccctgccctt tttctttaaa accgaaaaga ttacttcgcg ttatgcaggc ttcctcgctc
     1921 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     1981 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     2041 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca caggctccgc
     2101 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtgg
//
