LOCUS       sequence-471477-        2199 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  HA C-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_5a_005_HA-tag
FEATURES             Location/Qualifiers
     source          1..2199
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      223..811
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     712..731
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     965..982
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1006..1063)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     CDS             complement(1106..1132)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(1136..1162)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(1166..1192)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    1214..1235
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      1238..1281
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(1285..1303)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             1465..2124
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1531..1550)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      join(2145..2199,1..40)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 aacgctcggt tgccgccggg cgttttttat tggtgagaat ccaagcctcg agctgtcaga
       61 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
      121 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
      181 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
      241 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
      301 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
      361 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
      421 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
      481 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
      541 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
      601 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
      661 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
      721 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
      781 atgctcgtca ggggggcgga gcctgtggaa aaacgccagc aacgcggcct ttttacggtt
      841 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
      901 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
      961 gcgcagcgag tcagtgagcg aggaagcctg cataacgcga agtaatcttt tcggttttaa
     1021 agaaaaaggg cagggtggtg acaccttgcc cttttttgcc ggactgcagc ggccgctact
     1081 agtcgcgatg agtggtctca tacccagcat aatctggaac atcataagga taaccagcat
     1141 aatcaggaac atcgtatggg tattttgcgt aatctggtac atcatatgga tataaagccg
     1201 agaccactca tcgctctaga agcggccgcg aattccagaa atcatcctta gcgaaagcta
     1261 aggatttttt ttatctgaaa ttctgcctcg tgatacgcct atttttatag gttaatgtca
     1321 tgataataat ggtttcttag acgtcaggtg gcactcgagt tgatcgggca cgtaagaggt
     1381 tccaactttc accataatga aataagatca ctaccgggcg tattttttga gttatcgaga
     1441 ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat
     1501 atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc
     1561 tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag
     1621 cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa
     1681 tttcgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac
     1741 accgttttcc atgagcaaac tgaaacgttt tcatcactct ggagtgaata ccacgacgat
     1801 ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc
     1861 tatttcccta aagggtttat tgagaatatg tttttcgtat cagccaatcc ctgggtgagt
     1921 ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc
     1981 atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat
     2041 catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc
     2101 gacgagtggc agggcggggc gtaatttgat atcgagctcg cttggactcc tgttgataga
     2161 tccagtaatg acctcagaac tccatctgga tttgttcag
//
