LOCUS       sequence-471479-        2619 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nanoluc luciferase reporter gene, codon optimized for Chlamydomonas 
            reinhardtii.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_4_001_Nanoluc
FEATURES             Location/Qualifiers
     source          1..2619
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(6..23)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(177..765)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(257..276)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(948..1042)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1063..1722)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1637..1656
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1884..1902
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1906..1949)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    1952..1973
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      2544..2601
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
ORIGIN
        1 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
       61 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
      121 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
      181 cacaggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
      241 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
      301 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
      361 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
      421 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
      481 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
      541 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
      601 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
      661 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
      721 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
      781 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
      841 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
      901 atctaaagta tatatgagta aacttggtct gacagctcga ggcttggatt ctcaccaata
      961 aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca gatggagttc tgaggtcatt
     1021 actggatcta tcaacaggag tccaagcgag ctcgatatca aattacgccc cgccctgcca
     1081 ctcgtcgcag tactgttgta attcattaag cattctgccg acatggaagc catcacaaac
     1141 ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt
     1201 gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact
     1261 ggtgaaactc acccagggat tggctgatac gaaaaacata ttctcaataa accctttagg
     1321 gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaactg
     1381 ccggaaatcg tcgtggtatt cactccagag tgatgaaaac gtttcagttt gctcatggaa
     1441 aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt tcattgccat
     1501 acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa
     1561 cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct gaacggtctg
     1621 gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac gatgccattg
     1681 ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc
     1741 tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat tatggtgaaa
     1801 gttggaacct cttacgtgcc cgatcaactc gagtgccacc tgacgtctaa gaaaccatta
     1861 ttatcatgac attaacctat aaaaataggc gtatcacgag gcagaatttc agataaaaaa
     1921 aatccttagc tttcgctaag gatgatttct ggaattcgcg gccgcttcta gagcgatgag
     1981 tggtctcgaa tggttttcac tttagaagac ttcgtaggtg actggcgtca aacagcaggt
     2041 tataatttag accaagtttt agaacaaggt ggtgtatcaa gtttattcca aaatttaggt
     2101 gtttctgtta ctccaattca acgtatcgta ttaagtggtg aaaatggtct taaaattgac
     2161 atccatgtta ttattcctta tgaaggtctt tcaggtgacc aaatgggtca aattgaaaaa
     2221 atttttaaag tagtttatcc tgtagatgac catcacttta aagtaatttt acactatggt
     2281 actttagtaa ttgatggcgt tacacctaat atgattgact actttggtcg tccttatgaa
     2341 ggtattgctg tttttgatgg taaaaaaatc acagttacag gtacattatg gaatggtaat
     2401 aaaattattg acgaacgttt aatcaatcct gatggttcat tattattccg tgttacaatt
     2461 aatggtgtta caggttggcg tctttgtgaa cgtatccttg ctgctttgag accactcatc
     2521 gcgactagta gcggccgctg cagtccggca aaaaagggca aggtgtcacc accctgccct
     2581 ttttctttaa aaccgaaaag attacttcgc gttatgcag
//
