LOCUS       sequence-471480-        2802 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mScarlet-I reporter gene, codon optimized for Chlamydomonas 
            reinhardtii.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_4_007_mScarlet-I
FEATURES             Location/Qualifiers
     source          1..2802
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(135..229)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(250..909)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     824..843
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1071..1089
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1093..1136)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    1139..1160
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      1914..1971
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1995..2012)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2166..2754)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2246..2265)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
       61 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
      121 agctcgaggc ttggattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac
      181 aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc
      241 gatatcaaat tacgccccgc cctgccactc gtcgcagtac tgttgtaatt cattaagcat
      301 tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag
      361 caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc
      421 catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg ctgatacgaa
      481 aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac
      541 atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagtga
      601 tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat
      661 caccagctca ccgtctttca ttgccatacg aaattccgga tgagcattca tcaggcgggc
      721 aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa
      781 ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc
      841 ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt
      901 tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg
      961 tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgcccga tcaactcgag
     1021 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     1081 tcacgaggca gaatttcaga taaaaaaaat ccttagcttt cgctaaggat gatttctgga
     1141 attcgcggcc gcttctagag cgatgagtgg tctcgaatgg tttcaaaagg tgaagcagta
     1201 attaaagaat ttatgcgttt caaagttcat atggaaggtt ctatgaatgg tcacgaattt
     1261 gaaattgaag gtgaaggtga aggtcgtcca tatgaaggta cacaaactgc taaattaaaa
     1321 gtaacaaaag gtggtccatt accttttagt tgggatattt tatctccaca atttatgtat
     1381 ggttcacgtg catttattaa acacccagca gacattccag attactataa acaatcattc
     1441 ccagaaggtt tcaaatggga acgtgttatg aactttgaag atggtggtgc agttactgta
     1501 acacaagata catcattaga agatggtact ttaatttata aagttaaatt acgtggtaca
     1561 aattttccac ctgatggtcc tgtaatgcaa aaaaaaacaa tgggttggga agcttcaact
     1621 gaacgtttat atcctgaaga tggtgtatta aaaggtgata ttaaaatggc attacgttta
     1681 aaagatggtg gtcgttattt agctgacttt aaaacaactt ataaagcaaa aaaacctgtt
     1741 caaatgcctg gtgcttataa cgttgatcgt aaattagaca ttacttcaca caatgaagat
     1801 tatacagtag ttgaacaata tgaacgtagt gaaggtcgtc attctacagg tggtatggat
     1861 gaattatata aagctttgag accactcatc gcgactagta gcggccgctg cagtccggca
     1921 aaaaagggca aggtgtcacc accctgccct ttttctttaa aaccgaaaag attacttcgc
     1981 gttatgcagg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     2041 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     2101 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     2161 gcgtttttcc acaggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     2221 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     2281 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     2341 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     2401 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     2461 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     2521 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     2581 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     2641 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     2701 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     2761 cctttgatct tttctacggg gtctgacgct cagtggaacg aa
//
