LOCUS       sequence-471481-        3534 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  tdTomato reporter gene, codon optimized for Chlamydomonas 
            reinhardtii.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 3534)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_4_008_tdTomato
FEATURES             Location/Qualifiers
     source          1..3534
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      613..707
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      890..1478
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1379..1398
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1632..1649
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1673..1730)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    3216..3237
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      3240..3283
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(3287..3305)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             join(3467..3534,1..592)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(join(3533..3534,1..18))
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc
       61 ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct
      121 tgcccgcctg atgaatgctc atccggaatt tcgtatggca atgaaagacg gtgagctggt
      181 gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc
      241 atcactctgg agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga
      301 tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt
      361 tttcgtatca gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat
      421 ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt
      481 gctgatgccg ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag
      541 aatgcttaat gaattacaac agtactgcga cgagtggcag ggcggggcgt aatttgatat
      601 cgagctcgct tggactcctg ttgatagatc cagtaatgac ctcagaactc catctggatt
      661 tgttcagaac gctcggttgc cgccgggcgt tttttattgg tgagaatcca agcctcgagc
      721 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
      781 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
      841 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
      901 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
      961 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     1021 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     1081 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     1141 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     1201 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     1261 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     1321 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     1381 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     1441 ttttgtgatg ctcgtcaggg gggcggagcc tgtggaaaaa cgccagcaac gcggcctttt
     1501 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     1561 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     1621 cgaccgagcg cagcgagtca gtgagcgagg aagcctgcat aacgcgaagt aatcttttcg
     1681 gttttaaaga aaaagggcag ggtggtgaca ccttgccctt ttttgccgga ctgcagcggc
     1741 cgctactagt cgcgatgagt ggtctcaaag ctttgtataa ttcgtccata ccatataaga
     1801 ataaatggtg acgaccttct gaacgttcgt attgttcaac aattgtgtaa tcttcattat
     1861 gtgatgtaat gtctaatttt gtatctacat agtaataacc aggtaattga acaggttttt
     1921 tagccatgta aattgtttta aattcaacta aatagtgacc accatctttt aattttaaag
     1981 cttgatgaat ttcacctttt aatacaccgt cacgtgggta taaacgttca gtagaagctt
     2041 cccaacccat tgtttttttt tgcataacag gaccatcagg tgggaagttt gtaccacgca
     2101 ttttaacttt ataaattaaa gtaccatctt gtaaagatga atcttgagtt actgtaacta
     2161 aaccaccatc ttcaaaattc attacacgtt cccatttgaa accttctgga aaacttaatt
     2221 ttttataatc aggaatatca gctggatgtt taacataagc tttagaacca tacatgaatt
     2281 gtggtgataa aatatcccat gcgaaaggta atggaccacc tttagttact tttaattttg
     2341 cagtttgagt accttcatat ggacgacctt caccttcacc ttcaatttcg aattcatgac
     2401 cattcataga accttccata cgtactttaa aacgcataaa ttctttaatt actgccatgt
     2461 tattatcttc acttgaagca gtaccagatg aacctgaacc tgtagaacca gtaccgtgac
     2521 ctaaaaataa atggtgacga ccttcactac gttcatattg ttctacaata gtgtaatctt
     2581 cattgtgaga agtaatgtct aatttagtat caacatagta ataaccaggt aattgtacag
     2641 gtttttttgc catataaatt gttttaaatt caactaaata gtgaccacca tcttttaatt
     2701 ttaatgcttg gtgaatttca ccttttaaaa caccatcacg tggatataaa cgttctgtac
     2761 ttgcttccca acccatagtt tttttttgca taactggacc atcaggtgga aaattagtac
     2821 cacgcatttt aactttataa attaatgtac catcttgtaa acttgagtct tgagtaactg
     2881 taactaaacc accatcttcg aaattcatta cacgttccca tttaaaacct tctggaaaac
     2941 ttaatttttt ataatctgga atgtctgctg gatgttttac ataagctttt gaaccgtaca
     3001 taaattgtgg acttaaaata tcccaagcga atggtaatgg accaccttta gtaactttta
     3061 attttgctgt ttgtgtacct tcatatggac gaccttcacc ttcaccttca atttcaaatt
     3121 catgaccatt catagaacct tccatacgta ctttaaaacg catgaattct ttaattactt
     3181 cttcaccttt tgaaaccatt cgagaccact catcgctcta gaagcggccg cgaattccag
     3241 aaatcatcct tagcgaaagc taaggatttt ttttatctga aattctgcct cgtgatacgc
     3301 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactcga
     3361 gttgatcggg cacgtaagag gttccaactt tcaccataat gaaataagat cactaccggg
     3421 cgtatttttt gagttatcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat
     3481 cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt ttga
//
