LOCUS       sequence-471483-        3387 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cytosine deaminase selection gene, codon optimized for Chlamydomonas
            reinhardtii.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_4_024_CrCD-marker
FEATURES             Location/Qualifiers
     source          1..3387
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      540..634
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      817..1405
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1306..1325
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1559..1576
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1600..1657)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    2996..3017
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      3020..3063
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(3067..3085)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             join(3247..3387,1..519)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3313..3332)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg
       61 aatgctcatc cggaatttcg tatggcaatg aaagacggtg agctggtgat atgggatagt
      121 gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc actctggagt
      181 gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac
      241 ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt cgtatcagcc
      301 aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc
      361 gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg
      421 gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa
      481 ttacaacagt actgcgacga gtggcagggc ggggcgtaat ttgatatcga gctcgcttgg
      541 actcctgttg atagatccag taatgacctc agaactccat ctggatttgt tcagaacgct
      601 cggttgccgc cgggcgtttt ttattggtga gaatccaagc ctcgagctgt cagaccaagt
      661 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
      721 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
      781 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
      841 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
      901 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
      961 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     1021 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     1081 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     1141 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     1201 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     1261 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     1321 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     1381 gtcagggggg cggagcctgt ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     1441 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     1501 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     1561 cgagtcagtg agcgaggaag cctgcataac gcgaagtaat cttttcggtt ttaaagaaaa
     1621 agggcagggt ggtgacacct tgcccttttt tgccggactg cagcggccgc tactagtcgc
     1681 gatgagtggt ctcaaagcac gtttgtagtc aatagcttct ggttgttcta aataaacagt
     1741 tgtttgagct ggttgagttg aagcaataac tttaccacca cgaactgagt aacgtactgg
     1801 aacttgacga cgtaaagcat cgaaaccgtt ttcagctggt aaaataatta agttagctga
     1861 gttaccagca gcaataccgt agtcttgtaa gtttaaagta cgagcagagt ggtgagtaat
     1921 taagtttaaa ccgtcgttaa tttgaccgta acccattaat tgacatacgt gtaaacccat
     1981 gtgtaaaact tgtaacatgt tagcagtacc taaagggtac caaggaccac aaacgtcgtc
     2041 gtgaccaaaa caaacattaa tacctgattc taacatttct ttaacacgtg taataccacg
     2101 acgttttggg taagtatcga aacgaccttg taagtgaatg tttactaatg ggttagcaac
     2161 gaagttaata ccagacattt ttaataaacg gaataaacga gaagtgtaag caccgttgta
     2221 agagtgcata gctgtagtgt gtgaagcagt aacacgagca cccatacctt cgtggtgagc
     2281 taaagcagct actgtttcaa cgaaacgtga ttgttcgtca tcaatttcgt cacagtgaac
     2341 atcaattaaa cgatcatatt tttgagctaa agcaaatgtt ttgtgtaaag attcaacacc
     2401 atattcacgt gtaaattcga agtgtggaat agcaccaaca acatcagcac ctaaacgtaa
     2461 agcttcttct aataaagctt caccgtttgg gtatgataaa ataccttctt gtgggaaagc
     2521 agcaatttgt aagtcaatcc atggagctac ttcttgttta acttctaaca tagcttttaa
     2581 agctgttaaa gtagcatcag aaacgtctac gtgagtacgt acgtgttgaa taccgttagc
     2641 aatttgccat tttaatgttt gccaagcacg ttgtttaacg tcgtcgtgtg ttaataaagc
     2701 tttacgttca gcccaacgct caataccttc aaataaagta cctgattggt tccagtttgg
     2761 ttgaccagct gtttgtgtag tatctaagtg aatgtgtggt tcaacgaatg gtggaataac
     2821 taaaccttgt tcagcatcta aagagttttc agtaattggc attacaccag attgagcatc
     2881 aatagctgaa attttaccgt cttgtaagtg aatttgccat aaaccttctt cacctggtaa
     2941 acgagcgtta ataattgttt gtaaagcgtt gttagacatt cgagaccact catcgctcta
     3001 gaagcggccg cgaattccag aaatcatcct tagcgaaagc taaggatttt ttttatctga
     3061 aattctgcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     3121 agacgtcagg tggcactcga gttgatcggg cacgtaagag gttccaactt tcaccataat
     3181 gaaataagat cactaccggg cgtatttttt gagttatcga gattttcagg agctaaggaa
     3241 gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt
     3301 aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag
     3361 ctggatatta cggccttttt aaagacc
//
