LOCUS       sequence-471484-        3486 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  N-acetyl ornithine aminotransferase selection gene, codon optimized 
            for Chlamydomonas reinhardtii.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 3486)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_4_026_Arg9-marker
FEATURES             Location/Qualifiers
     source          1..3486
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     96..113
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(137..194)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    1632..1653
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      1656..1699
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(1703..1721)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             1883..2542
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1949..1968)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      2563..2657
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      2840..3428
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3329..3348
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
       61 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcct
      121 gcataacgcg aagtaatctt ttcggtttta aagaaaaagg gcagggtggt gacaccttgc
      181 ccttttttgc cggactgcag cggccgctac tagtcgcgat gagtggtctc aaagcttttg
      241 gagcaacttt atttaaagct gcagctaaaa cttcacaagc agtatcaatt tcagcgtctg
      301 taactactaa aggtggaact aaacgaataa catcaccttt acctgcagta attgccataa
      361 cacccatatc acgagctgca tcaactactg gacctgccat catatctaat tgtacaccaa
      421 ctaataaacc aacaccacga acttcttgta catgagggtt accagccatt gtacgacgta
      481 aaccagcacg taaacgttca cctttagctt caactgcagc taaaaaagct ggtgagttaa
      541 taatatcaaa tactgaacaa gctacatgac atactaaagg attacctgca aatgtactac
      601 cgtgatcacc aggtttcatt acatcagcaa cgtgttgttt taataaaact gtaccaatag
      661 gtaaaccacc agctaatggt ttagctaatg tcatcatgtc tggttctaca ccgaataatt
      721 ggtgacccca taatttacct gtacgaccta aaccacattg tacttcatca aatactaata
      781 aagcaccagc ttcatcacat aattgacgta aaccttttaa aaatgcttgt gtagatggtg
      841 taacaccacc ttcaccttga acaggttcta caaaaacagc tgctgtttta ccttttttaa
      901 taactgcagc tgcactttct aagttattat attctgctaa ttgatgacca ggcatcattg
      961 gatagaatgg tgttttatat tgttctttat atgttaatgc taaagcaccc attgtacgac
     1021 cgtgaaaaca tgatgtaaat gatactaatt catatggagc tactgcacca ccatcatatg
     1081 ggtcaatacc agcacgtaca cgagcccatt tacgagcgaa tttaatagca ccttcatttg
     1141 cttctgtacc tgtattacaa aagaaagctt tgtctgcgaa actattttct actaaacgtt
     1201 ttgctaattc aacttgtggt tgtgtgtggt ataaatttga tgtatgagct aatttttcag
     1261 cttgttctac taaagctgca taccattgtg aatctgagtg acctaatgca ttaactgcaa
     1321 tacctgcagc catatcaata tattctttac cttctacgtc ccacatttta gcacctttac
     1381 cgtgagaaat tacaactgga gcacgaccat atgtttgtaa aacatattta gattcatcag
     1441 caataacttg tttagtgaaa gctgcatctt taggtgttgg tttacttgaa gttaaacctt
     1501 tgaataatga agcagggttg aatgttaatg cagcacgagg agcaacagct aattgacgag
     1561 cacgtttagg ttgagctgca gctgcacgac cttgatgttg ttgtaattgt gccattcgag
     1621 accactcatc gctctagaag cggccgcgaa ttccagaaat catccttagc gaaagctaag
     1681 gatttttttt atctgaaatt ctgcctcgtg atacgcctat ttttataggt taatgtcatg
     1741 ataataatgg tttcttagac gtcaggtggc actcgagttg atcgggcacg taagaggttc
     1801 caactttcac cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt
     1861 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
     1921 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
     1981 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
     2041 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
     2101 tcgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
     2161 cgttttccat gagcaaactg aaacgttttc atcactctgg agtgaatacc acgacgattt
     2221 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta
     2281 tttccctaaa gggtttattg agaatatgtt tttcgtatca gccaatccct gggtgagttt
     2341 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat
     2401 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
     2461 tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
     2521 cgagtggcag ggcggggcgt aatttgatat cgagctcgct tggactcctg ttgatagatc
     2581 cagtaatgac ctcagaactc catctggatt tgttcagaac gctcggttgc cgccgggcgt
     2641 tttttattgg tgagaatcca agcctcgagc tgtcagacca agtttactca tatatacttt
     2701 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     2761 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     2821 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     2881 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     2941 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     3001 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     3061 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     3121 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     3181 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     3241 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     3301 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     3361 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     3421 tgtggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     3481 ctcaca
//
