LOCUS       sequence-471487-        2191 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3'UTR dummy sequence used as a baseline in 3'UTR characterization.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_B_0_5_012_3'UTR_dummy
FEATURES             Location/Qualifiers
     source          1..2191
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(43..62)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(734..828)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(849..1508)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1423..1442
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1670..1688
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1692..1735)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    1738..1759
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      1902..1959
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1983..2000)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(2154..2191,1..551))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
       61 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
      121 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
      181 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
      241 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
      301 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
      361 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
      421 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
      481 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
      541 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
      601 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
      661 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
      721 gctcgaggct tggattctca ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca
      781 aatccagatg gagttctgag gtcattactg gatctatcaa caggagtcca agcgagctcg
      841 atatcaaatt acgccccgcc ctgccactcg tcgcagtact gttgtaattc attaagcatt
      901 ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc
      961 accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc
     1021 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgatacgaaa
     1081 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca
     1141 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagtgat
     1201 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc
     1261 accagctcac cgtctttcat tgccatacga aattccggat gagcattcat caggcgggca
     1321 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag
     1381 gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc
     1441 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt
     1501 ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt
     1561 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgcccgat caactcgagt
     1621 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     1681 cacgaggcag aatttcagat aaaaaaaatc cttagctttc gctaaggatg atttctggaa
     1741 ttcgcggccg cttctagagc gatgagtggt ctcggcttaa actcagttgt agtaacgagc
     1801 ggatagattc ccagacccac cttcacgggc ggtagcagga cctcaataat aggattttcg
     1861 cgcttgagac cactcatcgc gactagtagc ggccgctgca gtccggcaaa aaagggcaag
     1921 gtgtcaccac cctgcccttt ttctttaaaa ccgaaaagat tacttcgcgt tatgcaggct
     1981 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     2041 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     2101 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccac
     2161 aggctccgcc cccctgacga gcatcacaaa a
//
