LOCUS       sequence-471488-        2231 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  psbA 3'UTR Chlamydomonas reinhardtii.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_5_001_psbA_3'UTR_Cr
FEATURES             Location/Qualifiers
     source          1..2231
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(63..82)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(754..848)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(869..1528)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1443..1462
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1690..1708
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1712..1755)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    1758..1779
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      1962..2019
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(2043..2060)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
       61 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
      121 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
      181 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
      241 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
      301 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
      361 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
      421 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
      481 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
      541 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
      601 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
      661 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
      721 tgagtaaact tggtctgaca gctcgaggct tggattctca ccaataaaaa acgcccggcg
      781 gcaaccgagc gttctgaaca aatccagatg gagttctgag gtcattactg gatctatcaa
      841 caggagtcca agcgagctcg atatcaaatt acgccccgcc ctgccactcg tcgcagtact
      901 gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca tgatgaacct
      961 gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc atggtgaaaa
     1021 cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg aaactcaccc
     1081 agggattggc tgatacgaaa aacatattct caataaaccc tttagggaaa taggccaggt
     1141 tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt
     1201 ggtattcact ccagagtgat gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag
     1261 ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacga aattccggat
     1321 gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg tgcttatttt
     1381 tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta taggtacatt
     1441 gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat atatcaacgg
     1501 tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgata
     1561 actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg gaacctctta
     1621 cgtgcccgat caactcgagt gccacctgac gtctaagaaa ccattattat catgacatta
     1681 acctataaaa ataggcgtat cacgaggcag aatttcagat aaaaaaaatc cttagctttc
     1741 gctaaggatg atttctggaa ttcgcggccg cttctagagc gatgagtggt ctcggcttaa
     1801 ggatcctttt tttttaaact aaaataaatc tggttaacca tacctggttt attttagttt
     1861 atacacactt ttcatatata tatacttaat agctaccata ggcagttggc aggacgtccc
     1921 cgcttgagac cactcatcgc gactagtagc ggccgctgca gtccggcaaa aaagggcaag
     1981 gtgtcaccac cctgcccttt ttctttaaaa ccgaaaagat tacttcgcgt tatgcaggct
     2041 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     2101 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     2161 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccac
     2221 aggctccgcc c
//
