LOCUS       sequence-471489-        2331 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  rrnB 3'UTR Chlamydomonas reinhardtii.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_5_005_rrnB_3'UTR_Cr
FEATURES             Location/Qualifiers
     source          1..2331
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(36..130)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(151..810)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     725..744
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     972..990
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(994..1037)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    1040..1061
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      complement(1094..1121)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1213..1299)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1344..1401
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1425..1442)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1596..2184)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1676..1695)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tatgagtaaa cttggtctga cagctcgagg cttggattct caccaataaa aaacgcccgg
       61 cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac tggatctatc
      121 aacaggagtc caagcgagct cgatatcaaa ttacgccccg ccctgccact cgtcgcagta
      181 ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg catgatgaac
      241 ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa
      301 aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac
      361 ccagggattg gctgatacga aaaacatatt ctcaataaac cctttaggga aataggccag
      421 gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc
      481 gtggtattca ctccagagtg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca
      541 agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac gaaattccgg
      601 atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt
      661 tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca
      721 ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac
      781 ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga
      841 taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaacctct
      901 tacgtgcccg atcaactcga gtgccacctg acgtctaaga aaccattatt atcatgacat
      961 taacctataa aaataggcgt atcacgaggc agaatttcag ataaaaaaaa tccttagctt
     1021 tcgctaagga tgatttctgg aattcgcggc cgcttctaga gcgatgagtg gtctcggctt
     1081 aagtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttaattt gatgcctggc
     1141 agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcgcaa cgttcaaatc
     1201 cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac agataaaacg
     1261 aaaggcccag tctttcgact gagcctttcg ttttatttga tgcgcttgag accactcatc
     1321 gcgactagta gcggccgctg cagtccggca aaaaagggca aggtgtcacc accctgccct
     1381 ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct cactgactcg
     1441 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     1501 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     1561 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc acaggctccg cccccctgac
     1621 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     1681 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     1741 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     1801 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     1861 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     1921 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     1981 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     2041 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     2101 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     2161 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     2221 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     2281 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat a
//
