LOCUS       sequence-471490-        2204 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  psbA 3'UTR Nicotiana tabacum.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_5_006_psbA_3'UTR_Nt
FEATURES             Location/Qualifiers
     source          1..2204
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     187..204
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(228..285)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    441..462
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      465..508
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(512..530)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             692..1351
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(758..777)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1372..1466
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     2138..2157
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatgctcgt caggggggcg gagcctgtgg aaaaacgcca gcaacgcggc ctttttacgg
       61 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
      121 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
      181 gagcgcagcg agtcagtgag cgaggaagcc tgcataacgc gaagtaatct tttcggtttt
      241 aaagaaaaag ggcagggtgg tgacaccttg cccttttttg ccggactgca gcggccgcta
      301 ctagtcgcga tgagtggtct caagcgaaaa aaagaaagga gcaatagcac cctcttgata
      361 gaacaagaaa atgattattg ctcctttctt ttcaaaacct cctatagact aggccaggat
      421 taagccgaga ccactcatcg ctctagaagc ggccgcgaat tccagaaatc atccttagcg
      481 aaagctaagg atttttttta tctgaaattc tgcctcgtga tacgcctatt tttataggtt
      541 aatgtcatga taataatggt ttcttagacg tcaggtggca ctcgagttga tcgggcacgt
      601 aagaggttcc aactttcacc ataatgaaat aagatcacta ccgggcgtat tttttgagtt
      661 atcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac
      721 cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca
      781 atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa
      841 aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca
      901 tccggaattt cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc
      961 ttgttacacc gttttccatg agcaaactga aacgttttca tcactctgga gtgaatacca
     1021 cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa
     1081 cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtatcag ccaatccctg
     1141 ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt
     1201 tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca
     1261 ggttcatcat gccgtttgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca
     1321 gtactgcgac gagtggcagg gcggggcgta atttgatatc gagctcgctt ggactcctgt
     1381 tgatagatcc agtaatgacc tcagaactcc atctggattt gttcagaacg ctcggttgcc
     1441 gccgggcgtt ttttattggt gagaatccaa gcctcgagct gtcagaccaa gtttactcat
     1501 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     1561 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     1621 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     1681 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     1741 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     1801 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     1861 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     1921 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     1981 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     2041 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     2101 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     2161 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttg
//
