LOCUS       sequence-471491-        2510 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  atpA 3'UTR Chlamydomonas reinhardtii.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_5_007_atpA_3'UTR_Cr
FEATURES             Location/Qualifiers
     source          1..2510
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(122..710)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(202..221)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(893..987)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1008..1667)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1582..1601
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1829..1847
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1851..1894)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    1897..1918
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      2380..2437
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(2461..2478)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
       61 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      121 ttttccacag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      181 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      241 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      301 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      361 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      421 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      481 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      541 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
      601 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      661 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      721 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      781 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
      841 aatcaatcta aagtatatat gagtaaactt ggtctgacag ctcgaggctt ggattctcac
      901 caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa atccagatgg agttctgagg
      961 tcattactgg atctatcaac aggagtccaa gcgagctcga tatcaaatta cgccccgccc
     1021 tgccactcgt cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca
     1081 caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa
     1141 tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca
     1201 aaactggtga aactcaccca gggattggct gatacgaaaa acatattctc aataaaccct
     1261 ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga
     1321 aactgccgga aatcgtcgtg gtattcactc cagagtgatg aaaacgtttc agtttgctca
     1381 tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt
     1441 gccatacgaa attccggatg agcattcatc aggcgggcaa gaatgtgaat aaaggccgga
     1501 taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg
     1561 gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc tttacgatgc
     1621 cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta
     1681 gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg
     1741 tgaaagttgg aacctcttac gtgcccgatc aactcgagtg ccacctgacg tctaagaaac
     1801 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggcaga atttcagata
     1861 aaaaaaatcc ttagctttcg ctaaggatga tttctggaat tcgcggccgc ttctagagcg
     1921 atgagtggtc tcggcttaat tttaattaag taggaactcg gtatatgctc ttttggggtc
     1981 ttattagcta gtattagtta actaacaaaa gatcaatatt ttagtttgtt ttatatattt
     2041 tattacttaa gtagtaagga tttgcattta gcaatcttaa atacttaagt aataatctat
     2101 aaataaaata tattttcgct ttaaaactta taaaaattat ttgctcgtta taagcctaaa
     2161 aaaacgtagg atctctacga gatattacat tgtttttttc tttaattggc tttaatatta
     2221 ctttgtatat ataaaccaaa gtacttgtta atagttatta aattatatta actatacagt
     2281 acaaagaaat tttttgctaa aaaaagtatg ttaacattaa aaatttttgt ttatacagcg
     2341 cttgagacca ctcatcgcga ctagtagcgg ccgctgcagt ccggcaaaaa agggcaaggt
     2401 gtcaccaccc tgcccttttt ctttaaaacc gaaaagatta cttcgcgtta tgcaggcttc
     2461 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
//
