LOCUS       sequence-471492-        2197 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  atpB 3'UTR Chlamydomonas reinhardtii.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_5_010_atpB_3'UTR_Cr
FEATURES             Location/Qualifiers
     source          1..2197
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(53..147)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(168..827)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     742..761
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     989..1007
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1011..1054)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    1057..1078
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      1227..1284
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1308..1325)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1479..2067)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1559..1578)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aatcaatcta aagtatatat gagtaaactt ggtctgacag ctcgaggctt ggattctcac
       61 caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa atccagatgg agttctgagg
      121 tcattactgg atctatcaac aggagtccaa gcgagctcga tatcaaatta cgccccgccc
      181 tgccactcgt cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca
      241 caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa
      301 tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca
      361 aaactggtga aactcaccca gggattggct gatacgaaaa acatattctc aataaaccct
      421 ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga
      481 aactgccgga aatcgtcgtg gtattcactc cagagtgatg aaaacgtttc agtttgctca
      541 tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt
      601 gccatacgaa attccggatg agcattcatc aggcgggcaa gaatgtgaat aaaggccgga
      661 taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg
      721 gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc tttacgatgc
      781 cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta
      841 gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg
      901 tgaaagttgg aacctcttac gtgcccgatc aactcgagtg ccacctgacg tctaagaaac
      961 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggcaga atttcagata
     1021 aaaaaaatcc ttagctttcg ctaaggatga tttctggaat tcgcggccgc ttctagagcg
     1081 atgagtggtc tcggcttaat ttaatttttt atgtgtaatt aaaataaata tttggacacc
     1141 attaagttgt tttcttctta aagagccaat ttattttaat tacaccgctt gagaccactc
     1201 atcgcgacta gtagcggccg ctgcagtccg gcaaaaaagg gcaaggtgtc accaccctgc
     1261 cctttttctt taaaaccgaa aagattactt cgcgttatgc aggcttcctc gctcactgac
     1321 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     1381 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     1441 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccacaggct ccgcccccct
     1501 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     1561 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     1621 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     1681 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     1741 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     1801 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     1861 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     1921 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     1981 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     2041 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     2101 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     2161 ttcacctaga tccttttaaa ttaaaaatga agtttta
//
