LOCUS       sequence-471493-        2459 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ftsH 3'UTR Chlamydomonas reinhardtii.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_5_013_ftsH_3'UTR_Cr
FEATURES             Location/Qualifiers
     source          1..2459
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    441..462
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      465..508
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(512..530)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             692..1351
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(758..777)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1372..1466
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      1649..2237
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2138..2157
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2391..2408
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(join(2432..2459,1..30))
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
ORIGIN
        1 ggtggtgaca ccttgccctt ttttgccgga ctgcagcggc cgctactagt cgcgatgagt
       61 ggtctcaagc ggggtaacta aagtttatcg aacttgccac catgagtgta ccattgctta
      121 accctcgtca ttataatttg caagttattc aatttttttg tccatgagca gtataaccat
      181 gaaatggctt acttttattg gtgttgttta cgctatcgtg cagcccttat atctacagac
      241 cctaatttta ctctttctcg gggaagataa tttattaggg atatagatat ggttagttgg
      301 acgttcctta cagggctaca caataacgaa gcggagtgag agtaactagc acaagaaata
      361 gtattcacat ggtgcagtta gaagctgccg tatgttttat taatactgtt taaatattat
      421 taagccgaga ccactcatcg ctctagaagc ggccgcgaat tccagaaatc atccttagcg
      481 aaagctaagg atttttttta tctgaaattc tgcctcgtga tacgcctatt tttataggtt
      541 aatgtcatga taataatggt ttcttagacg tcaggtggca ctcgagttga tcgggcacgt
      601 aagaggttcc aactttcacc ataatgaaat aagatcacta ccgggcgtat tttttgagtt
      661 atcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac
      721 cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca
      781 atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa
      841 aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca
      901 tccggaattt cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc
      961 ttgttacacc gttttccatg agcaaactga aacgttttca tcactctgga gtgaatacca
     1021 cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa
     1081 cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtatcag ccaatccctg
     1141 ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt
     1201 tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca
     1261 ggttcatcat gccgtttgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca
     1321 gtactgcgac gagtggcagg gcggggcgta atttgatatc gagctcgctt ggactcctgt
     1381 tgatagatcc agtaatgacc tcagaactcc atctggattt gttcagaacg ctcggttgcc
     1441 gccgggcgtt ttttattggt gagaatccaa gcctcgagct gtcagaccaa gtttactcat
     1501 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     1561 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     1621 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     1681 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     1741 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     1801 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     1861 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     1921 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     1981 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     2041 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     2101 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     2161 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     2221 ggcggagcct gtggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     2281 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     2341 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     2401 tgagcgagga agcctgcata acgcgaagta atcttttcgg ttttaaagaa aaagggcag
//
