LOCUS       sequence-471494-        2310 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  petD 3'UTR Chlamydomonas reinhardtii.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_5_017_petD_3'UTR_Cr
FEATURES             Location/Qualifiers
     source          1..2310
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1..95
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      278..866
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     767..786
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1020..1037
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1061..1118)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    1380..1401
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      1404..1447
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(1451..1469)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             1631..2290
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1697..1716)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gactcctgtt gatagatcca gtaatgacct cagaactcca tctggatttg ttcagaacgc
       61 tcggttgccg ccgggcgttt tttattggtg agaatccaag cctcgagctg tcagaccaag
      121 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
      181 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
      241 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
      301 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
      361 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
      421 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
      481 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
      541 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
      601 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
      661 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
      721 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
      781 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
      841 cgtcaggggg gcggagcctg tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
      901 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
      961 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     1021 gcgagtcagt gagcgaggaa gcctgcataa cgcgaagtaa tcttttcggt tttaaagaaa
     1081 aagggcaggg tggtgacacc ttgccctttt ttgccggact gcagcggccg ctactagtcg
     1141 cgatgagtgg tctcaagcgg ttgtaactaa gttatttgtt tggtttaaat taaatggaat
     1201 ctcctatttt gtaggatgaa caagggtgtt atttacccgc attatctgct aagacacaat
     1261 ttattgatgg agttcttaaa aaaatgacag aactcagttt tcccccttca gggttgcaaa
     1321 tcgtattgca accctagagg gaaatttaaa attttagatt aagccgagac cactcatcgc
     1381 tctagaagcg gccgcgaatt ccagaaatca tccttagcga aagctaagga ttttttttat
     1441 ctgaaattct gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     1501 tcttagacgt caggtggcac tcgagttgat cgggcacgta agaggttcca actttcacca
     1561 taatgaaata agatcactac cgggcgtatt ttttgagtta tcgagatttt caggagctaa
     1621 ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca
     1681 tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt
     1741 tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc
     1801 ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaatttc gtatggcaat
     1861 gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga
     1921 gcaaactgaa acgttttcat cactctggag tgaataccac gacgatttcc ggcagtttct
     1981 acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg
     2041 gtttattgag aatatgtttt tcgtatcagc caatccctgg gtgagtttca ccagttttga
     2101 tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta
     2161 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga
     2221 tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgacg agtggcaggg
     2281 cggggcgtaa tttgatatcg agctcgcttg
//
