LOCUS       sequence-471495-        2217 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  psbA 3'UTR Chlamydomonas reinhardtii.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_5_021_psbA_3'UTR_Cr
FEATURES             Location/Qualifiers
     source          1..2217
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     181..198
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(222..279)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    448..469
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      472..515
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(519..537)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             699..1358
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(765..784)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1379..1473
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     2145..2164
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcgtcagggg ggcggagcct gtggaaaaac gccagcaacg cggccttttt acggttcctg
       61 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
      121 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
      181 agcgagtcag tgagcgagga agcctgcata acgcgaagta atcttttcgg ttttaaagaa
      241 aaagggcagg gtggtgacac cttgcccttt tttgccggac tgcagcggcc gctactagtc
      301 gcgatgagtg gtctcaagcg ctgccaactg cctatggtag ctattaagta tatatatatg
      361 aaaagtgtgt ataaactaaa ataaaccagg tatggttaac cagatttatt ttagtttaaa
      421 aaaaaattaa gccgagacca ctcatcgctc tagaagcggc cgcgaattcc agaaatcatc
      481 cttagcgaaa gctaaggatt ttttttatct gaaattctgc ctcgtgatac gcctattttt
      541 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactc gagttgatcg
      601 ggcacgtaag aggttccaac tttcaccata atgaaataag atcactaccg ggcgtatttt
      661 ttgagttatc gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat
      721 ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag
      781 ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg
      841 taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga
      901 atgctcatcc ggaatttcgt atggcaatga aagacggtga gctggtgata tgggatagtg
      961 ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatca ctctggagtg
     1021 aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg
     1081 gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtatcagcca
     1141 atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg
     1201 cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg
     1261 cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg cttaatgaat
     1321 tacaacagta ctgcgacgag tggcagggcg gggcgtaatt tgatatcgag ctcgcttgga
     1381 ctcctgttga tagatccagt aatgacctca gaactccatc tggatttgtt cagaacgctc
     1441 ggttgccgcc gggcgttttt tattggtgag aatccaagcc tcgagctgtc agaccaagtt
     1501 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     1561 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     1621 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     1681 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     1741 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     1801 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     1861 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     1921 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     1981 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     2041 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     2101 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     2161 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgc
//
