LOCUS       sequence-471496-        2256 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  psbC 3'UTR Chlamydomonas reinhardtii.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_5_022_psbC_3'UTR_Cr
FEATURES             Location/Qualifiers
     source          1..2256
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(82..176)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(197..856)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     771..790
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1018..1036
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1040..1083)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    1086..1107
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      1315..1372
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1396..1413)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1567..2155)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1647..1666)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
       61 gtctgacagc tcgaggcttg gattctcacc aataaaaaac gcccggcggc aaccgagcgt
      121 tctgaacaaa tccagatgga gttctgaggt cattactgga tctatcaaca ggagtccaag
      181 cgagctcgat atcaaattac gccccgccct gccactcgtc gcagtactgt tgtaattcat
      241 taagcattct gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg
      301 gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga
      361 agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg
      421 atacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac
      481 acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc
      541 agagtgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat
      601 cccatatcac cagctcaccg tctttcattg ccatacgaaa ttccggatga gcattcatca
      661 ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct
      721 ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact
      781 gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag
      841 tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata
      901 cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg tgcccgatca
      961 actcgagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
     1021 aggcgtatca cgaggcagaa tttcagataa aaaaaatcct tagctttcgc taaggatgat
     1081 ttctggaatt cgcggccgct tctagagcga tgagtggtct cggcttaatt tttattatta
     1141 ttaaaattgg gttgttacca tgtaacaacc caattttaca actatcagat ttgttattta
     1201 ctaacaaacc attgaaatcg ctcatctgac acggaaaatg ccaatctttt taataacttt
     1261 atagcatttt ctacgcttga gaccactcat cgcgactagt agcggccgct gcagtccggc
     1321 aaaaaagggc aaggtgtcac caccctgccc tttttcttta aaaccgaaaa gattacttcg
     1381 cgttatgcag gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     1441 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     1501 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     1561 ggcgtttttc cacaggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     1621 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     1681 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     1741 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     1801 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     1861 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     1921 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     1981 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     2041 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     2101 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     2161 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     2221 tttggtcatg agattatcaa aaaggatctt caccta
//
