LOCUS       sequence-471497-        2178 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  rbcL 3'UTR Chlamydomonas reinhardtii.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_5_026_rbcL_3'UTR_Cr
FEATURES             Location/Qualifiers
     source          1..2178
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      212..800
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     701..720
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     954..971
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(995..1052)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    1182..1203
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      1206..1249
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(1253..1271)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             1433..2092
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1499..1518)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      join(2113..2178,1..29)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 gccgccgggc gttttttatt ggtgagaatc caagcctcga gctgtcagac caagtttact
       61 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
      121 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
      181 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
      241 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
      301 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
      361 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
      421 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
      481 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
      541 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
      601 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
      661 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
      721 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
      781 gggggcggag cctgtggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
      841 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
      901 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
      961 cagtgagcga ggaagcctgc ataacgcgaa gtaatctttt cggttttaaa gaaaaagggc
     1021 agggtggtga caccttgccc ttttttgccg gactgcagcg gccgctacta gtcgcgatga
     1081 gtggtctcaa gcgaacctaa acaccataaa aataaaaacg atgtttatgc tattcacata
     1141 aacatcatga aaaataaaaa ttaagccgag accactcatc gctctagaag cggccgcgaa
     1201 ttccagaaat catccttagc gaaagctaag gatttttttt atctgaaatt ctgcctcgtg
     1261 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     1321 actcgagttg atcgggcacg taagaggttc caactttcac cataatgaaa taagatcact
     1381 accgggcgta ttttttgagt tatcgagatt ttcaggagct aaggaagcta aaatggagaa
     1441 aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga
     1501 ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc
     1561 ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct
     1621 tgcccgcctg atgaatgctc atccggaatt tcgtatggca atgaaagacg gtgagctggt
     1681 gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc
     1741 atcactctgg agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga
     1801 tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt
     1861 tttcgtatca gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat
     1921 ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt
     1981 gctgatgccg ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag
     2041 aatgcttaat gaattacaac agtactgcga cgagtggcag ggcggggcgt aatttgatat
     2101 cgagctcgct tggactcctg ttgatagatc cagtaatgac ctcagaactc catctggatt
     2161 tgttcagaac gctcggtt
//
