LOCUS       sequence-471498-        2370 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  rps2 3'UTR Chlamydomonas reinhardtii.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_5_032_rps2_3'UTR_Cr
FEATURES             Location/Qualifiers
     source          1..2370
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     104..121
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(145..202)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    524..545
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      548..591
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(595..613)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             775..1434
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(841..860)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1455..1549
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      1732..2320
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2221..2240
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
       61 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
      121 ggaagcctgc ataacgcgaa gtaatctttt cggttttaaa gaaaaagggc agggtggtga
      181 caccttgccc ttttttgccg gactgcagcg gccgctacta gtcgcgatga gtggtctcaa
      241 gcgggaatag tttgcatatt aagtatataa atactaatca aaaagtagat atacccaacg
      301 aggatattaa aaagaatatc ctcgttgggt atatctactt gaatggcaga ggctgaacca
      361 actgcctcct tcggagtatt aaaataggat gttaatactg cggagcaggc agtggcggta
      421 ccactgccac taatatttat attcccgtaa ggggacgtcc tagaatgaca actaaaaaaa
      481 aactaaacca tatggttagt atttaagccg agaccactca tcgctctaga agcggccgcg
      541 aattccagaa atcatcctta gcgaaagcta aggatttttt ttatctgaaa ttctgcctcg
      601 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
      661 gcactcgagt tgatcgggca cgtaagaggt tccaactttc accataatga aataagatca
      721 ctaccgggcg tattttttga gttatcgaga ttttcaggag ctaaggaagc taaaatggag
      781 aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt
      841 gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg
      901 gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt
      961 cttgcccgcc tgatgaatgc tcatccggaa tttcgtatgg caatgaaaga cggtgagctg
     1021 gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt
     1081 tcatcactct ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa
     1141 gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg
     1201 tttttcgtat cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat
     1261 atggacaact tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag
     1321 gtgctgatgc cgctggcgat tcaggttcat catgccgttt gtgatggctt ccatgtcggc
     1381 agaatgctta atgaattaca acagtactgc gacgagtggc agggcggggc gtaatttgat
     1441 atcgagctcg cttggactcc tgttgataga tccagtaatg acctcagaac tccatctgga
     1501 tttgttcaga acgctcggtt gccgccgggc gttttttatt ggtgagaatc caagcctcga
     1561 gctgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     1621 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     1681 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     1741 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     1801 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     1861 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     1921 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     1981 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     2041 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     2101 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     2161 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     2221 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     2281 atttttgtga tgctcgtcag gggggcggag cctgtggaaa aacgccagca acgcggcctt
     2341 tttacggttc ctggcctttt gctggccttt
//
