LOCUS       sequence-471499-        2489 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  rps8 3'UTR Chlamydomonas reinhardtii.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_5_035_rps8_3'UTR_Cr
FEATURES             Location/Qualifiers
     source          1..2489
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(39..58)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(730..824)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(845..1504)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1419..1438
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1666..1684
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1688..1731)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    1734..1755
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      2196..2253
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(2277..2294)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(2448..2489,1..547))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
       61 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
      121 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
      181 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
      241 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
      301 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
      361 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
      421 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
      481 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
      541 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
      601 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
      661 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagctc
      721 gaggcttgga ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc
      781 cagatggagt tctgaggtca ttactggatc tatcaacagg agtccaagcg agctcgatat
      841 caaattacgc cccgccctgc cactcgtcgc agtactgttg taattcatta agcattctgc
      901 cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct
      961 tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat
     1021 tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgat acgaaaaaca
     1081 tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt
     1141 gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agtgatgaaa
     1201 acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca
     1261 gctcaccgtc tttcattgcc atacgaaatt ccggatgagc attcatcagg cgggcaagaa
     1321 tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg
     1381 taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa
     1441 aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg atttttttct
     1501 ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg
     1561 atcttatttc attatggtga aagttggaac ctcttacgtg cccgatcaac tcgagtgcca
     1621 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     1681 aggcagaatt tcagataaaa aaaatcctta gctttcgcta aggatgattt ctggaattcg
     1741 cggccgcttc tagagcgatg agtggtctcg gcttaatttt ttataaattt aaaactggct
     1801 acctctgtaa aaatatttaa tatggcatca ccagaggtac cagaaatgta tttacctttc
     1861 ctcccaaaaa cacttatttt aaaatctaat tattgacaga agttgacctt gtaaatatta
     1921 cttaaattaa tttagcagta tgtcaattta gttagttata gtaccacttt gggagagggt
     1981 attacattaa tttattttga aatttactta tagtaaatag agatttggga acaacaactg
     2041 ctagcctacc gatttacaaa acgtggtttt aataatttat taattggaag gcgactgcaa
     2101 aagaatattt tgcagcatag gatttatttc taatttttct aaagttacca attgcgcttg
     2161 agaccactca tcgcgactag tagcggccgc tgcagtccgg caaaaaaggg caaggtgtca
     2221 ccaccctgcc ctttttcttt aaaaccgaaa agattacttc gcgttatgca ggcttcctcg
     2281 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     2341 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     2401 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccacaggctc
     2461 cgcccccctg acgagcatca caaaaatcg
//
