LOCUS       sequence-471500-        2307 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  rps11 3'UTR Chlamydomonas reinhardtii.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_5_036_rps11_3'UTR_Cr
FEATURES             Location/Qualifiers
     source          1..2307
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(108..202)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(223..882)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     797..816
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1044..1062
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1066..1109)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    1112..1133
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      1392..1449
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1473..1490)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1644..2232)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1724..1743)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
       61 atctaaagta tatatgagta aacttggtct gacagctcga ggcttggatt ctcaccaata
      121 aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca gatggagttc tgaggtcatt
      181 actggatcta tcaacaggag tccaagcgag ctcgatatca aattacgccc cgccctgcca
      241 ctcgtcgcag tactgttgta attcattaag cattctgccg acatggaagc catcacaaac
      301 ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt
      361 gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact
      421 ggtgaaactc acccagggat tggctgatac gaaaaacata ttctcaataa accctttagg
      481 gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaactg
      541 ccggaaatcg tcgtggtatt cactccagag tgatgaaaac gtttcagttt gctcatggaa
      601 aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt tcattgccat
      661 acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa
      721 cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct gaacggtctg
      781 gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac gatgccattg
      841 ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc
      901 tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat tatggtgaaa
      961 gttggaacct cttacgtgcc cgatcaactc gagtgccacc tgacgtctaa gaaaccatta
     1021 ttatcatgac attaacctat aaaaataggc gtatcacgag gcagaatttc agataaaaaa
     1081 aatccttagc tttcgctaag gatgatttct ggaattcgcg gccgcttcta gagcgatgag
     1141 tggtctcggc ttaattttta aaaaattaaa gatataccaa tttatttttt gtatacaagt
     1201 tttatagtgc aatattttta taaaatattt ccttgattat gcttgagttt tagtatttta
     1261 ccatgctcat gtcaaccata aacttgtata caaaacatta acaatttttt atttattgaa
     1321 attgaaattt atttctattt aaaaatgcag cgcttgagac cactcatcgc gactagtagc
     1381 ggccgctgca gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt ttctttaaaa
     1441 ccgaaaagat tacttcgcgt tatgcaggct tcctcgctca ctgactcgct gcgctcggtc
     1501 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     1561 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     1621 aaaaaggccg cgttgctggc gtttttccac aggctccgcc cccctgacga gcatcacaaa
     1681 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     1741 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     1801 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     1861 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     1921 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     1981 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     2041 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     2101 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     2161 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     2221 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     2281 aactcacgtt aagggatttt ggtcatg
//
