LOCUS       sequence-471502-        2332 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  tufA 3'UTR Chlamydomonas reinhardtii.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_5_039_tufA_3'UTR_Cr
FEATURES             Location/Qualifiers
     source          1..2332
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      199..787
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     688..707
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     941..958
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(982..1039)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    1323..1344
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      1347..1390
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(1394..1412)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             1574..2233
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1640..1659)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      join(2254..2332,1..16)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 ttttattggt gagaatccaa gcctcgagct gtcagaccaa gtttactcat atatacttta
       61 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
      121 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
      181 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
      241 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
      301 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
      361 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
      421 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
      481 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
      541 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
      601 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
      661 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
      721 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
      781 gtggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
      841 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
      901 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
      961 agcctgcata acgcgaagta atcttttcgg ttttaaagaa aaagggcagg gtggtgacac
     1021 cttgcccttt tttgccggac tgcagcggcc gctactagtc gcgatgagtg gtctcaagcg
     1081 cccgaaggga acttttttca gagaatataa atataggatt ttaatactcc gaaggaggca
     1141 gtggcggtac cacaataaat aaatttgtcc tcacctatgg tcgaaccttt tcgggtaaat
     1201 aaatagcaaa ttcctgctta cgcagtatta tatcctaaca gagccttggc atcccaaagg
     1261 ggacgtccgt taagggctcg ttagtgaatt taaatgtgaa attaagccga gaccactcat
     1321 cgctctagaa gcggccgcga attccagaaa tcatccttag cgaaagctaa ggattttttt
     1381 tatctgaaat tctgcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     1441 gtttcttaga cgtcaggtgg cactcgagtt gatcgggcac gtaagaggtt ccaactttca
     1501 ccataatgaa ataagatcac taccgggcgt attttttgag ttatcgagat tttcaggagc
     1561 taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg
     1621 gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac
     1681 cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta
     1741 tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat ttcgtatggc
     1801 aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca
     1861 tgagcaaact gaaacgtttt catcactctg gagtgaatac cacgacgatt tccggcagtt
     1921 tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa
     1981 agggtttatt gagaatatgt ttttcgtatc agccaatccc tgggtgagtt tcaccagttt
     2041 tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca tgggcaaata
     2101 ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtttg
     2161 tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg acgagtggca
     2221 gggcggggcg taatttgata tcgagctcgc ttggactcct gttgatagat ccagtaatga
     2281 cctcagaact ccatctggat ttgttcagaa cgctcggttg ccgccgggcg tt
//
