LOCUS       sequence-471503-        2381 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ycf12 3'UTR Chlamydomonas reinhardtii.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_5_041_ycf12_3'UTR_Cr
FEATURES             Location/Qualifiers
     source          1..2381
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     99..116
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(140..197)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    530..551
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      554..597
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(601..619)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             781..1440
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(847..866)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1461..1555
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      1738..2326
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2227..2246
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
       61 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
      121 cctgcataac gcgaagtaat cttttcggtt ttaaagaaaa agggcagggt ggtgacacct
      181 tgcccttttt tgccggactg cagcggccgc tactagtcgc gatgagtggt ctcaagcggt
      241 tgatctccaa tattgtttct tagtcattaa ctttaaagat tgtcacaaac agcctttata
      301 tgaccataaa gatataaact tcgaatacat aataaatacg ttcaagaagc tgctatgcag
      361 attaaagtaa gctatttata agtgttaatg tgtgtttgtg aacaaaattt ttctttgtta
      421 ttaagaagta tgtatgtacc cgaaagtcta aacgtcactt tcaggtacat acattttata
      481 aggctagcaa ttgctatgct attttaaatt aagccgagac cactcatcgc tctagaagcg
      541 gccgcgaatt ccagaaatca tccttagcga aagctaagga ttttttttat ctgaaattct
      601 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
      661 caggtggcac tcgagttgat cgggcacgta agaggttcca actttcacca taatgaaata
      721 agatcactac cgggcgtatt ttttgagtta tcgagatttt caggagctaa ggaagctaaa
      781 atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa
      841 cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat
      901 attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt
      961 cacattcttg cccgcctgat gaatgctcat ccggaatttc gtatggcaat gaaagacggt
     1021 gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa
     1081 acgttttcat cactctggag tgaataccac gacgatttcc ggcagtttct acacatatat
     1141 tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag
     1201 aatatgtttt tcgtatcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg
     1261 gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc
     1321 gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga tggcttccat
     1381 gtcggcagaa tgcttaatga attacaacag tactgcgacg agtggcaggg cggggcgtaa
     1441 tttgatatcg agctcgcttg gactcctgtt gatagatcca gtaatgacct cagaactcca
     1501 tctggatttg ttcagaacgc tcggttgccg ccgggcgttt tttattggtg agaatccaag
     1561 cctcgagctg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     1621 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     1681 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     1741 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     1801 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     1861 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     1921 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     1981 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     2041 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     2101 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     2161 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     2221 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     2281 gcgtcgattt ttgtgatgct cgtcaggggg gcggagcctg tggaaaaacg ccagcaacgc
     2341 ggccttttta cggttcctgg ccttttgctg gccttttgct c
//
