LOCUS       sequence-471504-        2242 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BMV 3'UTR Brome Mosaic Virus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_5_042_BMV_3'UTR_Bv
FEATURES             Location/Qualifiers
     source          1..2242
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(68..87)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(759..853)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(874..1533)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1448..1467
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1695..1713
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1717..1760)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    1763..1784
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      1978..2035
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(2059..2076)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
       61 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
      121 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
      181 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
      241 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
      301 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
      361 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
      421 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
      481 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
      541 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
      601 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
      661 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
      721 atatatgagt aaacttggtc tgacagctcg aggcttggat tctcaccaat aaaaaacgcc
      781 cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct
      841 atcaacagga gtccaagcga gctcgatatc aaattacgcc ccgccctgcc actcgtcgca
      901 gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaaa cggcatgatg
      961 aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt
     1021 gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact
     1081 cacccaggga ttggctgata cgaaaaacat attctcaata aaccctttag ggaaataggc
     1141 caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc
     1201 gtcgtggtat tcactccaga gtgatgaaaa cgtttcagtt tgctcatgga aaacggtgta
     1261 acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca tacgaaattc
     1321 cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt
     1381 atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt
     1441 acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc
     1501 aacggtggta tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct
     1561 cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc
     1621 tcttacgtgc ccgatcaact cgagtgccac ctgacgtcta agaaaccatt attatcatga
     1681 cattaaccta taaaaatagg cgtatcacga ggcagaattt cagataaaaa aaatccttag
     1741 ctttcgctaa ggatgatttc tggaattcgc ggccgcttct agagcgatga gtggtctcgg
     1801 cttaaggtgc ctttgagagt ctacttttgc tctcttcgga agaaccctta ggggttcgtg
     1861 catgggcttg catagcaagt ctagatgcgg gtaccgtaca gtgttgaaaa acactgtaaa
     1921 tctctaaaag aaaccacgct tgagaccact catcgcgact agtagcggcc gctgcagtcc
     1981 ggcaaaaaag ggcaaggtgt caccaccctg ccctttttct ttaaaaccga aaagattact
     2041 tcgcgttatg caggcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     2101 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     2161 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     2221 gctggcgttt ttccacaggc tc
//
