LOCUS       sequence-471505-        2251 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  rbcL 3'UTR Nicotiana tabacum.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_5_043_rbcL_3'UTR_Nt
FEATURES             Location/Qualifiers
     source          1..2251
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(73..92)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(764..858)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(879..1538)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1453..1472
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1700..1718
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1722..1765)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    1768..1789
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      1992..2049
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(2073..2090)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
       61 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
      121 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
      181 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
      241 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
      301 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
      361 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
      421 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
      481 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
      541 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
      601 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
      661 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
      721 aaagtatata tgagtaaact tggtctgaca gctcgaggct tggattctca ccaataaaaa
      781 acgcccggcg gcaaccgagc gttctgaaca aatccagatg gagttctgag gtcattactg
      841 gatctatcaa caggagtcca agcgagctcg atatcaaatt acgccccgcc ctgccactcg
      901 tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca
      961 tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc
     1021 atggtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg
     1081 aaactcaccc agggattggc tgatacgaaa aacatattct caataaaccc tttagggaaa
     1141 taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg
     1201 aaatcgtcgt ggtattcact ccagagtgat gaaaacgttt cagtttgctc atggaaaacg
     1261 gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacga
     1321 aattccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg
     1381 tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta
     1441 taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat
     1501 atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa
     1561 aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg
     1621 gaacctctta cgtgcccgat caactcgagt gccacctgac gtctaagaaa ccattattat
     1681 catgacatta acctataaaa ataggcgtat cacgaggcag aatttcagat aaaaaaaatc
     1741 cttagctttc gctaaggatg atttctggaa ttcgcggccg cttctagagc gatgagtggt
     1801 ctcggcttaa aaacagtaga cattagcaga taaattagca ggaaataaag aaggataagg
     1861 agaaagaact caagtaatta tccttcgttc tcttaattga attgcaatta aactcggccc
     1921 aatcttttac taaaaggatt gagccgaata cgcttgagac cactcatcgc gactagtagc
     1981 ggccgctgca gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt ttctttaaaa
     2041 ccgaaaagat tacttcgcgt tatgcaggct tcctcgctca ctgactcgct gcgctcggtc
     2101 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     2161 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     2221 aaaaaggccg cgttgctggc gtttttccac a
//
