LOCUS       sequence-471506-        2298 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  rpoA 3'UTR Chlamydomonas reinhardtii.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_5_045_rpoA_3'UTR_Cr
FEATURES             Location/Qualifiers
     source          1..2298
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      272..860
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     761..780
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1014..1031
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1055..1112)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    1362..1383
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      1386..1429
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(1433..1451)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             1613..2272
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1679..1698)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      join(2293..2298,1..89)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 tgttgataga tccagtaatg acctcagaac tccatctgga tttgttcaga acgctcggtt
       61 gccgccgggc gttttttatt ggtgagaatc caagcctcga gctgtcagac caagtttact
      121 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
      181 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
      241 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
      301 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
      361 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
      421 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
      481 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
      541 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
      601 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
      661 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
      721 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
      781 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
      841 gggggcggag cctgtggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
      901 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
      961 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1021 cagtgagcga ggaagcctgc ataacgcgaa gtaatctttt cggttttaaa gaaaaagggc
     1081 agggtggtga caccttgccc ttttttgccg gactgcagcg gccgctacta gtcgcgatga
     1141 gtggtctcaa gcgattttta aattgattca attgtgaaat aacacgacat gtgtatctag
     1201 ggaatagttt cttcaaagcg aattctccct agatacatct attcaattta attctgaatt
     1261 tattttgaat atatgatata ttaatatatt aattgtgcta aagagtttca atctattttc
     1321 actaagtaag tccaatagat ttaagccgag accactcatc gctctagaag cggccgcgaa
     1381 ttccagaaat catccttagc gaaagctaag gatttttttt atctgaaatt ctgcctcgtg
     1441 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     1501 actcgagttg atcgggcacg taagaggttc caactttcac cataatgaaa taagatcact
     1561 accgggcgta ttttttgagt tatcgagatt ttcaggagct aaggaagcta aaatggagaa
     1621 aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga
     1681 ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc
     1741 ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct
     1801 tgcccgcctg atgaatgctc atccggaatt tcgtatggca atgaaagacg gtgagctggt
     1861 gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc
     1921 atcactctgg agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga
     1981 tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt
     2041 tttcgtatca gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat
     2101 ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt
     2161 gctgatgccg ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag
     2221 aatgcttaat gaattacaac agtactgcga cgagtggcag ggcggggcgt aatttgatat
     2281 cgagctcgct tggactcc
//
