LOCUS       sequence-471507-        2216 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  psbA 3'UTR Chlamydomonas reinhardtii.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_5b_001_psbA_3'UTR_Cr
FEATURES             Location/Qualifiers
     source          1..2216
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     181..198
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(222..279)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    447..468
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      471..514
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(518..536)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             698..1357
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(764..783)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1378..1472
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     2144..2163
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcgtcagggg ggcggagcct gtggaaaaac gccagcaacg cggccttttt acggttcctg
       61 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
      121 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
      181 agcgagtcag tgagcgagga agcctgcata acgcgaagta atcttttcgg ttttaaagaa
      241 aaagggcagg gtggtgacac cttgcccttt tttgccggac tgcagcggcc gctactagtc
      301 gcgatgagtg gtctcaagcg ctgccaactg cctatggtag ctattaagta tatatatatg
      361 aaaagtgtgt ataaactaaa ataaaccagg tatggttaac cagatttatt ttagtttaaa
      421 aaaaaattac ccgagaccac tcatcgctct agaagcggcc gcgaattcca gaaatcatcc
      481 ttagcgaaag ctaaggattt tttttatctg aaattctgcc tcgtgatacg cctattttta
      541 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcactcg agttgatcgg
      601 gcacgtaaga ggttccaact ttcaccataa tgaaataaga tcactaccgg gcgtattttt
      661 tgagttatcg agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata
      721 taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt
      781 tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt
      841 aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa
      901 tgctcatccg gaatttcgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt
      961 tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcac tctggagtga
     1021 ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg
     1081 tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg tatcagccaa
     1141 tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc
     1201 ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc
     1261 gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt
     1321 acaacagtac tgcgacgagt ggcagggcgg ggcgtaattt gatatcgagc tcgcttggac
     1381 tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc agaacgctcg
     1441 gttgccgccg ggcgtttttt attggtgaga atccaagcct cgagctgtca gaccaagttt
     1501 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     1561 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     1621 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     1681 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     1741 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     1801 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     1861 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     1921 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     1981 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     2041 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     2101 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     2161 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgc
//
