LOCUS       sequence-471508-        2196 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  atpB 3'UTR Chlamydomonas reinhardtii.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_5b_002_atpB_3'UTR_Cr
FEATURES             Location/Qualifiers
     source          1..2196
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(45..64)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(736..830)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(851..1510)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1425..1444
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1672..1690
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1694..1737)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    1740..1761
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      1909..1966
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1990..2007)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(2161..2196,1..553))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
       61 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
      121 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
      181 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
      241 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
      301 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
      361 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
      421 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
      481 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
      541 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
      601 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
      661 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
      721 cagctcgagg cttggattct caccaataaa aaacgcccgg cggcaaccga gcgttctgaa
      781 caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc caagcgagct
      841 cgatatcaaa ttacgccccg ccctgccact cgtcgcagta ctgttgtaat tcattaagca
      901 ttctgccgac atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca
      961 gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt
     1021 ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgatacga
     1081 aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca
     1141 catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagtg
     1201 atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata
     1261 tcaccagctc accgtctttc attgccatac gaaattccgg atgagcattc atcaggcggg
     1321 caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa
     1381 aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg
     1441 cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt
     1501 ttttctccat tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg
     1561 gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgcccg atcaactcga
     1621 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     1681 atcacgaggc agaatttcag ataaaaaaaa tccttagctt tcgctaagga tgatttctgg
     1741 aattcgcggc cgcttctaga gcgatgagtg gtctcgggta atttaatttt ttatgtgtaa
     1801 ttaaaataaa tatttggaca ccattaagtt gttttcttct taaagagcca atttatttta
     1861 attacaccgc ttgagaccac tcatcgcgac tagtagcggc cgctgcagtc cggcaaaaaa
     1921 gggcaaggtg tcaccaccct gccctttttc tttaaaaccg aaaagattac ttcgcgttat
     1981 gcaggcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     2041 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     2101 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     2161 tttccacagg ctccgccccc ctgacgagca tcacaa
//
