LOCUS       sequence-471509-        2266 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  petL 3'UTR Chlamydomonas reinhardtii.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_5b_003_petL_3'UTR_Cr
FEATURES             Location/Qualifiers
     source          1..2266
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(80..99)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(771..865)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(886..1545)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1460..1479
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1707..1725
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1729..1772)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    1775..1796
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      2014..2071
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(2095..2112)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ttccacaggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
       61 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
      121 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
      181 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
      241 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
      301 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
      361 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
      421 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
      481 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
      541 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
      601 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
      661 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
      721 tcaatctaaa gtatatatga gtaaacttgg tctgacagct cgaggcttgg attctcacca
      781 ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc
      841 attactggat ctatcaacag gagtccaagc gagctcgata tcaaattacg ccccgccctg
      901 ccactcgtcg cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca
      961 aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata
     1021 tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa
     1081 actggtgaaa ctcacccagg gattggctga tacgaaaaac atattctcaa taaacccttt
     1141 agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa
     1201 ctgccggaaa tcgtcgtggt attcactcca gagtgatgaa aacgtttcag tttgctcatg
     1261 gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc
     1321 catacgaaat tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata
     1381 aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt
     1441 ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca
     1501 ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc
     1561 tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg
     1621 aaagttggaa cctcttacgt gcccgatcaa ctcgagtgcc acctgacgtc taagaaacca
     1681 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcagaat ttcagataaa
     1741 aaaaatcctt agctttcgct aaggatgatt tctggaattc gcggccgctt ctagagcgat
     1801 gagtggtctc gggtaattta ttaatgagta aaaccacgct aatgggtttt tactactaca
     1861 ttttttatat ataattgtaa gagcacagca acgtgctctt acattttccg gttatttaag
     1921 tacctaacta aattcaaata aatgcgttaa gtttgagact gcaaagctgc cacgcttgag
     1981 accactcatc gcgactagta gcggccgctg cagtccggca aaaaagggca aggtgtcacc
     2041 accctgccct ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct
     2101 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     2161 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     2221 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgttt
//
