LOCUS       sequence-471510-        2297 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TMV 3'UTR Tobacco Mosaic Virus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_5b_005_TMV_3'UTR_Tv
FEATURES             Location/Qualifiers
     source          1..2297
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     141..158
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(182..239)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    488..509
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      512..555
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(559..577)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             739..1398
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(805..824)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1419..1513
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      1696..2284
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2185..2204
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
       61 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
      121 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcctgcata acgcgaagta
      181 atcttttcgg ttttaaagaa aaagggcagg gtggtgacac cttgcccttt tttgccggac
      241 tgcagcggcc gctactagtc gcgatgagtg gtctcaagcg tgggccccta ccgggggtaa
      301 cggggggatt cgaacccctc gctttattac gtgcctgcgg atgtatatga accatataca
      361 tttgacccgc gcgatccaag acacaaccct tcgatttaag tggagggaaa aacactatgc
      421 gttatcgtac gcaccacgtg tgattacgga cacaatccgt tatttattta cccgagacca
      481 ctcatcgctc tagaagcggc cgcgaattcc agaaatcatc cttagcgaaa gctaaggatt
      541 ttttttatct gaaattctgc ctcgtgatac gcctattttt ataggttaat gtcatgataa
      601 taatggtttc ttagacgtca ggtggcactc gagttgatcg ggcacgtaag aggttccaac
      661 tttcaccata atgaaataag atcactaccg ggcgtatttt ttgagttatc gagattttca
      721 ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc
      781 caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac
      841 cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag
      901 ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc ggaatttcgt
      961 atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt
     1021 ttccatgagc aaactgaaac gttttcatca ctctggagtg aataccacga cgatttccgg
     1081 cagtttctac acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc
     1141 cctaaagggt ttattgagaa tatgtttttc gtatcagcca atccctgggt gagtttcacc
     1201 agttttgatt taaacgtggc caatatggac aacttcttcg cccccgtttt caccatgggc
     1261 aaatattata cgcaaggcga caaggtgctg atgccgctgg cgattcaggt tcatcatgcc
     1321 gtttgtgatg gcttccatgt cggcagaatg cttaatgaat tacaacagta ctgcgacgag
     1381 tggcagggcg gggcgtaatt tgatatcgag ctcgcttgga ctcctgttga tagatccagt
     1441 aatgacctca gaactccatc tggatttgtt cagaacgctc ggttgccgcc gggcgttttt
     1501 tattggtgag aatccaagcc tcgagctgtc agaccaagtt tactcatata tactttagat
     1561 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     1621 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     1681 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     1741 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     1801 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     1861 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     1921 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     1981 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     2041 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     2101 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     2161 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     2221 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctgtg
     2281 gaaaaacgcc agcaacg
//
