LOCUS       sequence-471511-        2458 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ftsH 3'UTR Chlamydomonas reinhardtii.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_5b_006_ftsH_3'UTR_Cr
FEATURES             Location/Qualifiers
     source          1..2458
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      75..169
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      352..940
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     841..860
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1094..1111
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1135..1192)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    1602..1623
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      1626..1669
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(1673..1691)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             join(1853..2458,1..54)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1919..1938)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 agaatgctta atgaattaca acagtactgc gacgagtggc agggcggggc gtaatttgat
       61 atcgagctcg cttggactcc tgttgataga tccagtaatg acctcagaac tccatctgga
      121 tttgttcaga acgctcggtt gccgccgggc gttttttatt ggtgagaatc caagcctcga
      181 gctgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
      241 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
      301 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
      361 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
      421 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
      481 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
      541 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
      601 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
      661 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
      721 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
      781 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
      841 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
      901 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
      961 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     1021 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     1081 aacgaccgag cgcagcgagt cagtgagcga ggaagcctgc ataacgcgaa gtaatctttt
     1141 cggttttaaa gaaaaagggc agggtggtga caccttgccc ttttttgccg gactgcagcg
     1201 gccgctacta gtcgcgatga gtggtctcaa gcggggtaac taaagtttat cgaacttgcc
     1261 accatgagtg taccattgct taaccctcgt cattataatt tgcaagttat tcaatttttt
     1321 tgtccatgag cagtataacc atgaaatggc ttacttttat tggtgttgtt tacgctatcg
     1381 tgcagccctt atatctacag accctaattt tactctttct cggggaagat aatttattag
     1441 ggatatagat atggttagtt ggacgttcct tacagggcta cacaataacg aagcggagtg
     1501 agagtaacta gcacaagaaa tagtattcac atggtgcagt tagaagctgc cgtatgtttt
     1561 attaatactg tttaaatatt attacccgag accactcatc gctctagaag cggccgcgaa
     1621 ttccagaaat catccttagc gaaagctaag gatttttttt atctgaaatt ctgcctcgtg
     1681 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     1741 actcgagttg atcgggcacg taagaggttc caactttcac cataatgaaa taagatcact
     1801 accgggcgta ttttttgagt tatcgagatt ttcaggagct aaggaagcta aaatggagaa
     1861 aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga
     1921 ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc
     1981 ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct
     2041 tgcccgcctg atgaatgctc atccggaatt tcgtatggca atgaaagacg gtgagctggt
     2101 gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc
     2161 atcactctgg agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga
     2221 tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt
     2281 tttcgtatca gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat
     2341 ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt
     2401 gctgatgccg ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggc
//
